Department of Oral and Maxillofacial Surgery, School and Hospital of Stomatology, Peking University, Beijing 100081, China; Center for Biomedical Materials and Tissue Engineering, Academy for Advanced Interdisciplinary Studies, Peking University, Beijing 100081, China.
Carbohydr Polym. 2014 Jan 30;101:36-9. doi: 10.1016/j.carbpol.2013.09.030. Epub 2013 Sep 17.
Realization of the full potential of human induced pluripotent stem cells (hiPSCs) in clinical applications requires development of well-defined conditions for their growth and differentiation. A novel fully defined polyvinyl alcohol/hyaluronan (PVA/HA) polysaccharide nanofiber was developed for hiPSCs culture in commercially available xeno-free, chemically defined medium. Vitronectin peptide (VP) was immobilized to PVA/HA nanofibers through NHS/EDC chemistry. The hiPSCs successfully grew and proliferated on the VP-decorated PVA/HA nanofibers, similar to those on Matrigel™. Such well-defined, xeno-free and safe nanofiber substrate that supports culture of hiPSCs will not only help to accelerate the translational perspectives of hiPSCs, but also provide a platform to investigate the cell-nanofiber interaction mechanisms that regulate stem cell proliferation and differentiation.
要充分发挥人类诱导多能干细胞（hiPSCs）在临床应用中的潜力，需要开发明确的生长和分化条件。本文开发了一种新型的完全定义的聚乙烯醇/透明质酸（PVA/HA）多糖纳米纤维，用于在商业上可获得的无动物成分、化学定义的培养基中培养 hiPSCs。通过 NHS/EDC 化学将纤连蛋白肽（VP）固定在 PVA/HA 纳米纤维上。hiPSCs 可成功地在 VP 修饰的 PVA/HA 纳米纤维上生长和增殖，与 Matrigel™上的增殖情况类似。这种明确的、无动物成分的、安全的纳米纤维基质不仅有助于加速 hiPSCs 的转化研究，还为研究调节干细胞增殖和分化的细胞-纳米纤维相互作用机制提供了一个平台。
