用于检测澳大利亚牛泌尿生殖道拭子中解脲脲原体的聚合酶链反应
Polymerase chain reaction for detection of Ureaplasma diversum from urogenital swabs in cattle in Australia.
作者信息
Smith A, Chousalkar K K, Chenoweth P C
机构信息
School of Animal and Veterinary Science, University of Adelaide, Roseworthy, South Australia 5173, Australia.
出版信息
Aust Vet J. 2012 Jul;90(7):275-6. doi: 10.1111/j.1751-0813.2012.00939.x. Epub 2012 May 31.
BACKGROUND
Ureaplasma diversum has been associated with various reproductive problems in cattle, including granular vulvovaginitis, endometritis, salpingitis, early embryonic death, weak calves, decreased conception rates, balanoprosthitis, impaired spermatozoids and seminal vesiculitis in bulls.
METHODS
This study briefly outlines the use of polymerase chain reaction (PCR) for the rapid detection of U. diversum directly from urogenital swabs collected from Australian beef cattle.
RESULTS
The 16S ribosomal RNA gene sequences obtained from the PCR products of the clinical samples were closely related to U. diversum strain A417.
CONCLUSION
The present test enabled detection of the organism directly from clinical swabs collected from animals with or without lesions.
背景
牛支原体与牛的多种生殖问题有关，包括颗粒性外阴阴道炎、子宫内膜炎、输卵管炎、早期胚胎死亡、弱犊、受孕率降低、龟头包皮炎、公牛精子受损和精囊炎。
方法
本研究简要概述了使用聚合酶链反应（PCR）直接从澳大利亚肉牛采集的泌尿生殖道拭子中快速检测牛支原体的方法。
结果
从临床样本的PCR产物中获得的16S核糖体RNA基因序列与牛支原体A417菌株密切相关。
结论
本试验能够直接从有或无病变动物采集的临床拭子中检测到该病原体。
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