Effects of respiratory disease on Kele piglets lung microbiome, assessed through 16S rRNA sequencing.

BACKGROUND AND AIM

Due to the incomplete development of the immune system in immature piglets, the respiratory tract is susceptible to invasion by numerous pathogens that cause a range of potential respiratory diseases. However, few studies have reported the changes in pig lung microorganisms during respiratory infection. Therefore, we aimed to explore the differences in lung environmental microorganisms between healthy piglets and piglets with respiratory diseases.

MATERIALS AND METHODS

Histopathological changes in lung sections were observed in both diseased and healthy pigs. Changes in the composition and abundance of microbiomes in alveolar lavage fluid from eleven 4-week-old Chinese Kele piglets (three clinically healthy and eight diseased) were studied by IonS5™ XL sequencing of the bacterial16S rRNA genes.

RESULTS

Histopathological sections showed that diseased pigs displayed more lung lesions than healthy pigs. Diseased piglets harbored lower bacterial operational taxonomic units, α-diversity, and bacterial community complexity in comparison to healthy piglets. Taxonomic composition analysis showed that in the diseased piglets, the majority of flora was composed of , , and ; while , , and were dominant in the control group. The abundance of was significantly higher in ill piglets (p<0.05), and the phylogenetic tree indicated that was clustered in , a conditional pathogen that has the potential to affect the swine respiratory system.

CONCLUSION

The results of this study show that the microbial species and structure of piglets' lungs were changed during respiratory tract infection. The finding of suggested that besides known pathogens such as and , unknown pathogens can exist in the respiratory system of diseased pigs and provide a potential basis for clinical treatment.