Kunkel M E, Powers D L, Hord N G
Department of Food Science, Clemson University, South Carolina 29634-0371.
J Am Coll Nutr. 1990 Dec;9(6):633-40. doi: 10.1080/07315724.1990.10720420.
Absorptiometric, histomorphometric, and chemical analyses of bones from growing rats fed diets with low (0.2%, w/w), marginal (0.4%, w/w), or adequate (0.8%, w/w) calcium (Ca) content with or without phytate were compared. Phytate was added to each diet in a molar ratio of 19:1 to calcium. Male weanling Sprague-Dawley rats were fed one of the six diets for 8 weeks. At the end of 8 weeks, rats were killed, and mandibles, femurs, and tibias were removed. Bone density profiles were determined on the mandibles and femurs using single photon absorptiometry. Femurs were also used for calcium and phosphorus analyses. Tibias were used for histomorphometric analyses. Bone density of the femurs and mandibles increased as dietary Ca increased. The only effect of phytate addition measured was in the 0.8% calcium diet, where density was lower in rats fed the phytate-containing 0.8% calcium diet. Femur calcium concentration also increased as dietary Ca increased and was unaffected by addition of phytate. Femur phosphorus concentration was unaffected by dietary Ca levels but was increased by 10% when phytate was added to the diet. Bone density values were highly correlated with bone calcium and phosphorus levels (r = 0.94). Rats fed the 0.2% calcium diets had 20% lower mineralized bone area and 20% larger medullary cavity area than rats fed the other diets. Bone densitometry appears to be useful for determining changes in bone occurring in growing rats fed low, marginal, and adequate levels of dietary Ca. Bone density values also correlated well with chemically determined calcium and phosphorus concentrations and with histomorphometric data.
比较了给生长中的大鼠喂食钙含量低（0.2%，w/w）、边缘水平（0.4%，w/w）或充足（0.8%，w/w）且添加或不添加植酸盐的日粮后，对其骨骼进行的吸收测量法、组织形态计量学和化学分析。以钙与植酸盐19:1的摩尔比向每种日粮中添加植酸盐。雄性断奶斯普拉格-道利大鼠喂食六种日粮之一，持续8周。在8周结束时，处死大鼠，取出下颌骨、股骨和胫骨。使用单光子吸收测量法测定下颌骨和股骨的骨密度分布。股骨也用于钙和磷的分析。胫骨用于组织形态计量学分析。随着日粮钙含量的增加，股骨和下颌骨的骨密度增加。所测量的添加植酸盐的唯一影响是在0.8%钙日粮中，喂食含植酸盐的0.8%钙日粮的大鼠骨密度较低。股骨钙浓度也随着日粮钙含量的增加而增加，且不受植酸盐添加的影响。股骨磷浓度不受日粮钙水平的影响，但当向日粮中添加植酸盐时增加了10%。骨密度值与骨钙和磷水平高度相关（r = 0.94）。喂食0.2%钙日粮的大鼠矿化骨面积比喂食其他日粮的大鼠低20%，髓腔面积大20%。骨密度测量法似乎有助于确定喂食低、边缘和充足水平日粮钙的生长大鼠骨骼中发生的变化。骨密度值也与化学测定的钙和磷浓度以及组织形态计量学数据相关性良好。
