Hanssens M, Keirse M J, Lijnen P J, Kucharski M J, Luyten C, Van Assche F A
Department of Obstetrics, University of Leuven, Belgium.
J Biochem Biophys Methods. 1990 Jul-Aug;21(2):103-13. doi: 10.1016/0165-022x(90)90058-k.
Immunoreactive measurements of Angiotensin II in plasma, relate to a variety of angiotensin peptides with different biological activities. A method is described to differentiate these individual angiotensin peptides. It involves extraction of the peptides from plasma by reversible adsorption to phenylsilyl silica cartridges, separation by an isocratic, ion pairing high-pressure liquid chromatography technique and measurement of the appropriate fractions by radioimmunoassay. In umbilical venous plasma molar concentrations of the smaller angiotensin fragments were found to range between 16 and 25% of the concentrations of the angiotensin II octapeptide. Because some angiotensin antisera show higher affinity for the smaller peptides than for the octapeptide, concentrations of angiotensin II, measured by radioimmunoassay, may be overestimated by up to 35% unless the various angiotensin peptides are adequately separated.
血浆中血管紧张素II的免疫反应性测量与多种具有不同生物活性的血管紧张素肽有关。本文描述了一种区分这些单个血管紧张素肽的方法。该方法包括通过可逆吸附到苯基硅烷基硅胶柱上从血浆中提取肽，采用等度离子对高压液相色谱技术进行分离，并通过放射免疫测定法测量相应的馏分。在脐静脉血浆中，发现较小血管紧张素片段的摩尔浓度在血管紧张素II八肽浓度的16%至25%之间。由于一些血管紧张素抗血清对较小肽的亲和力高于对八肽的亲和力，除非各种血管紧张素肽得到充分分离，否则通过放射免疫测定法测得的血管紧张素II浓度可能会被高估高达35%。
