血管紧张素肽的测定：高效液相色谱-放射免疫分析法
Measurement of Angiotensin Peptides: HPLC-RIA.
作者信息
Brosnihan K Bridget, Chappell Mark C
机构信息
Department of Surgery, Hypertension & Vascular Research, Cardiovascular Sciences Center, Wake Forest University School of Medicine, Medical Center Blvd, Winston-Salem, NC, 27157-1032, USA.
出版信息
Methods Mol Biol. 2017;1527:81-99. doi: 10.1007/978-1-4939-6625-7_7.
Abstract
The renin-angiotensin system (RAS) is a complex circulating and tissue-based system. There are multiple pathways for the formation and degradation of peptides. In order to understand the functions of the system, characterization of angiotensin peptides (products and substrates) is important. Radioimmunoassays with the requisite specificity and sensitivity have been developed to allow for the characterization and quantification of circulating and tissue angiotensins. Here, we describe the appropriate methods for collecting the tissue and blood, the extractions steps required to partially purify and remove larger molecular weight-interfering proteins from tissue and plasma, and the radioimmunoassay of three of the peptides of this system (Ang I, Ang II, and Ang-(1-7)), as well as the verification of immunoreactive identity for Ang II and Ang-(1-7) by combined high-performance liquid chromatography-RIA analysis.
摘要
肾素-血管紧张素系统（RAS）是一个复杂的循环和基于组织的系统。肽的形成和降解有多种途径。为了了解该系统的功能，对血管紧张素肽（产物和底物）进行表征很重要。已经开发出具有所需特异性和灵敏度的放射免疫测定法，以对循环和组织中的血管紧张素进行表征和定量。在此，我们描述了收集组织和血液的合适方法、从组织和血浆中部分纯化并去除较大分子量干扰蛋白所需的提取步骤、该系统三种肽（血管紧张素I、血管紧张素II和血管紧张素-(1-7)）的放射免疫测定，以及通过高效液相色谱-放射免疫分析联用对血管紧张素II和血管紧张素-(1-7)免疫反应性身份的验证。
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