Division of Nephrology and Hypertension, Case Western Reserve University and University Hospitals Case Medical Center, Cleveland, Ohio 44116, USA.
Clin Chim Acta. 2012 Sep 8;413(17-18):1359-63. doi: 10.1016/j.cca.2012.03.006. Epub 2012 Mar 30.
The enzyme-linked immunosorbent spot (ELISPOT) assay for interferon gamma has been available for more than twenty years and has been used for a number of applications, including the monitoring of T cell immunity in solid organ transplant recipients. Studies from single centers indicate that heightened T cell alloreactivity measured with this assay correlates with acute and chronic rejection and with poor long-term allograft function. The assay has been used not only to assess T cell reactivity after transplantation, but also as a tool for assessing immune risk prior to transplantation. Additional work is needed to validate the assay in larger multicenter clinical trials.
酶联免疫斑点（ELISPOT）检测干扰素 γ已经有二十多年的历史，已经被用于许多应用，包括监测实体器官移植受者的 T 细胞免疫。来自单一中心的研究表明，该检测方法测量的 T 细胞同种反应性与急性和慢性排斥反应以及不良的长期移植物功能相关。该检测不仅用于评估移植后的 T 细胞反应性，也用于评估移植前的免疫风险。需要更多的工作来在更大的多中心临床试验中验证该检测。
