Mps1 促进 Aurora B 的快速着丝粒积累。
Mps1 promotes rapid centromere accumulation of Aurora B.
机构信息
Department of Medical Oncology, University Medical Center Utrecht, Universiteitsweg 100, STR 2.129, 3584 CG Utrecht, The Netherlands.
出版信息
EMBO Rep. 2012 Sep;13(9):847-54. doi: 10.1038/embor.2012.93. Epub 2012 Jun 26.
Abstract
Aurora B localization to mitotic centromeres, which is required for proper chromosome alignment during mitosis, relies on Haspin-dependent histone H3 phosphorylation and on Bub1-dependent histone H2A phosphorylation--which interacts with Borealin through a Shugoshin (Sgo) intermediate. We demonstrate that Mps1 stimulates the latter recruitment axis. Mps1 activity enhances H2A-T120ph and is critical for Sgo1 recruitment to centromeres, thereby promoting Aurora B centromere recruitment in early mitosis. Importantly, chromosome biorientation defects caused by Mps1 inhibition are improved by restoring Aurora B centromere recruitment. As Mps1 kinetochore localization reciprocally depends on Aurora B, we propose that this Aurora B-Mps1 recruitment circuitry cooperates with the Aurora B-Haspin feedback loop to ensure rapid centromere accumulation of Aurora B at the onset of mitosis.
摘要
极光 B 向有丝分裂着丝粒的定位，这是有丝分裂过程中正确染色体排列所必需的，依赖于 Haspin 依赖性组蛋白 H3 磷酸化和 Bub1 依赖性组蛋白 H2A 磷酸化，这些磷酸化与通过 Shugoshin（Sgo）中间物与 Borealin 相互作用。我们证明 Mps1 刺激了后一个募集轴。Mps1 活性增强 H2A-T120ph，对 Sgo1 向着丝粒的募集至关重要，从而促进早期有丝分裂中极光 B 向着丝粒的募集。重要的是，通过恢复极光 B 着丝粒募集可以改善 Mps1 抑制引起的染色体双定向缺陷。由于 Mps1 动粒定位与极光 B 相互依赖，我们提出这种极光 B-Mps1 募集电路与极光 B-Haspin 反馈环合作，以确保在有丝分裂开始时快速积累极光 B 到着丝粒。
相似文献
1
Mps1 promotes rapid centromere accumulation of Aurora B.Mps1 促进 Aurora B 的快速着丝粒积累。
EMBO Rep. 2012 Sep;13(9):847-54. doi: 10.1038/embor.2012.93. Epub 2012 Jun 26.
2
Aurora B kinase is recruited to multiple discrete kinetochore and centromere regions in human cells.极光激酶 B 在人类细胞中被招募到多个离散的着丝粒和着丝点区域。
J Cell Biol. 2020 Mar 2;219(3). doi: 10.1083/jcb.201905144.
3
Chromosome segregation regulation in human zygotes: altered mitotic histone phosphorylation dynamics underlying centromeric targeting of the chromosomal passenger complex.人类受精卵中染色体分离的调控：着丝粒靶向染色体乘客复合物的有丝分裂组蛋白磷酸化动力学改变。
Hum Reprod. 2015 Oct;30(10):2275-91. doi: 10.1093/humrep/dev186. Epub 2015 Jul 29.
4
A positive feedback loop involving Haspin and Aurora B promotes CPC accumulation at centromeres in mitosis.一个包含 Haspin 和 Aurora B 的正反馈环促进了有丝分裂中着丝粒处 CPC 的积累。
Curr Biol. 2011 Jun 21;21(12):1061-9. doi: 10.1016/j.cub.2011.05.016. Epub 2011 Jun 9.
5
Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.组蛋白 H3 Thr-3 的磷酸化由 Haspin 完成，使 Aurora B 在有丝分裂中定位于着丝粒。
Science. 2010 Oct 8;330(6001):231-5. doi: 10.1126/science.1189435. Epub 2010 Aug 12.
6
Two histone marks establish the inner centromere and chromosome bi-orientation.两种组蛋白标记建立了着丝粒内部和染色体的双定向。
Science. 2010 Oct 8;330(6001):239-43. doi: 10.1126/science.1194498.
7
TIP60 acetylation of Bub1 regulates centromeric H2AT120 phosphorylation for faithful chromosome segregation.TIP60 对 Bub1 的乙酰化调控着着丝粒区 H2AT120 的磷酸化，以保证染色体的正确分离。
Sci China Life Sci. 2024 Sep;67(9):1957-1969. doi: 10.1007/s11427-023-2604-8. Epub 2024 May 17.
8
A TPR domain-containing N-terminal module of MPS1 is required for its kinetochore localization by Aurora B.Aurora B 通过 MPS1 的 TPR 结构域包含的 N 端模块使其定位于动粒上。
J Cell Biol. 2013 Apr 15;201(2):217-31. doi: 10.1083/jcb.201210033. Epub 2013 Apr 8.
9
Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis.极光 B 增强 Mps1 的激活，以确保在有丝分裂开始时快速建立检查点。
Nat Commun. 2011;2:316. doi: 10.1038/ncomms1319.
10
A positive feedback mechanism ensures proper assembly of the functional inner centromere during mitosis in human cells.正反馈机制确保了人类细胞有丝分裂过程中功能性着丝粒内体的正确组装。
J Biol Chem. 2019 Feb 1;294(5):1437-1450. doi: 10.1074/jbc.RA118.006046. Epub 2018 Nov 29.
引用本文的文献
1
Proximity-based activation of AURORA A by MPS1 potentiates error correction.MPS1基于接近度激活极光激酶A可增强错误校正。
Curr Biol. 2025 Apr 21;35(8):1935-1947.e8. doi: 10.1016/j.cub.2025.03.018. Epub 2025 Apr 8.
2
Exploring the Therapeutic Implications of Co-Targeting the EGFR and Spindle Assembly Checkpoint Pathways in Oral Cancer.探索联合靶向表皮生长因子受体（EGFR）和纺锤体组装检查点通路在口腔癌中的治疗意义
Pharmaceutics. 2024 Sep 11;16(9):1196. doi: 10.3390/pharmaceutics16091196.
3
Sustained Shugoshin 1 downregulation reduces tumor growth and metastasis in a mouse xenograft tumor model of triple-negative breast cancer.持续下调守护蛋白1可减少三阴性乳腺癌小鼠异种移植瘤模型中的肿瘤生长和转移。
Cell Div. 2023 Apr 30;18(1):6. doi: 10.1186/s13008-023-00088-5.
4
Adaptation to high rates of chromosomal instability and aneuploidy through multiple pathways in budding yeast.在出芽酵母中，通过多种途径适应高染色体不稳定性和非整倍性。
EMBO J. 2023 Apr 17;42(8):e111500. doi: 10.15252/embj.2022111500. Epub 2022 Dec 19.
5
Mechanistic basis for Sgo1-mediated centromere localization and function of the CPC.Sgo1 介导的着丝粒定位的机制基础和 CPC 的功能。
J Cell Biol. 2022 Aug 1;221(8). doi: 10.1083/jcb.202108156. Epub 2022 Jul 1.
6
Aurora B activity is promoted by cooperation between discrete localization sites in budding yeast.极光 B 活性是由出芽酵母中离散定位点之间的合作促进的。
Mol Biol Cell. 2022 Aug 1;33(9):ar85. doi: 10.1091/mbc.E21-11-0590. Epub 2022 Jun 15.
7
Ginsenoside Rg1 suppresses cancer cell proliferation through perturbing mitotic progression.人参皂苷Rg1通过干扰有丝分裂进程抑制癌细胞增殖。
J Ginseng Res. 2022 May;46(3):481-488. doi: 10.1016/j.jgr.2021.11.004. Epub 2021 Nov 14.
8
The Role of Mitotic Kinases and the RZZ Complex in Kinetochore-Microtubule Attachments: Doing the Right Link.有丝分裂激酶和RZZ复合体在动粒-微管附着中的作用：建立正确连接
Front Cell Dev Biol. 2022 Jan 28;10:787294. doi: 10.3389/fcell.2022.787294. eCollection 2022.
9
Spatiotemporal coordination of the RSF1-PLK1-Aurora B cascade establishes mitotic signaling platforms.RSF1-PLK1-Aurora B 级联的时空协调建立了有丝分裂信号平台。
Nat Commun. 2021 Oct 11;12(1):5931. doi: 10.1038/s41467-021-26220-z.
10
BUB1 and CENP-U, Primed by CDK1, Are the Main PLK1 Kinetochore Receptors in Mitosis.在有丝分裂中，由 CDK1 引发的 BUB1 和 CENP-U 是 PLK1 动粒的主要受体。
Mol Cell. 2021 Jan 7;81(1):67-87.e9. doi: 10.1016/j.molcel.2020.10.040. Epub 2020 Nov 27.
本文引用的文献
1
Aurora B dynamics at centromeres create a diffusion-based phosphorylation gradient.着丝粒处极光 B 动力学形成基于扩散的磷酸化梯度。
J Cell Biol. 2011 Aug 22;194(4):539-49. doi: 10.1083/jcb.201103044. Epub 2011 Aug 15.
2
A positive feedback loop involving Haspin and Aurora B promotes CPC accumulation at centromeres in mitosis.一个包含 Haspin 和 Aurora B 的正反馈环促进了有丝分裂中着丝粒处 CPC 的积累。
Curr Biol. 2011 Jun 21;21(12):1061-9. doi: 10.1016/j.cub.2011.05.016. Epub 2011 Jun 9.
3
Aurora B potentiates Mps1 activation to ensure rapid checkpoint establishment at the onset of mitosis.极光 B 增强 Mps1 的激活，以确保在有丝分裂开始时快速建立检查点。
Nat Commun. 2011;2:316. doi: 10.1038/ncomms1319.
4
Evidence that Aurora B is implicated in spindle checkpoint signalling independently of error correction.有证据表明，Aurora B 独立于错误修正参与纺锤体检查点信号传导。
EMBO J. 2011 Apr 20;30(8):1508-19. doi: 10.1038/emboj.2011.70. Epub 2011 Mar 15.
5
Constitutive Mad1 targeting to kinetochores uncouples checkpoint signalling from chromosome biorientation.着丝粒定位的组成型 Mad1 使检验点信号从染色体的双定向中解耦。
Nat Cell Biol. 2011 Apr;13(4):475-82. doi: 10.1038/ncb2223. Epub 2011 Mar 13.
6
Bub1, Sgo1, and Mps1 mediate a distinct pathway for chromosome biorientation in budding yeast.Bub1、Sgo1 和 Mps1 介导了芽殖酵母中染色体的定向的一个独特途径。
Mol Biol Cell. 2011 May;22(9):1473-85. doi: 10.1091/mbc.E10-08-0673. Epub 2011 Mar 9.
7
Two histone marks establish the inner centromere and chromosome bi-orientation.两种组蛋白标记建立了着丝粒内部和染色体的双定向。
Science. 2010 Oct 8;330(6001):239-43. doi: 10.1126/science.1194498.
8
Phosphorylation of the CPC by Cdk1 promotes chromosome bi-orientation.Cdk1 对 CPC 的磷酸化促进了染色体的双定向。
Nature. 2010 Oct 7;467(7316):719-23. doi: 10.1038/nature09390. Epub 2010 Aug 25.
9
Survivin reads phosphorylated histone H3 threonine 3 to activate the mitotic kinase Aurora B.Survivin 通过读取磷酸化组蛋白 H3 丝氨酸 3 来激活有丝分裂激酶 Aurora B。
Science. 2010 Oct 8;330(6001):235-9. doi: 10.1126/science.1189505. Epub 2010 Aug 12.
10
Histone H3 Thr-3 phosphorylation by Haspin positions Aurora B at centromeres in mitosis.组蛋白 H3 Thr-3 的磷酸化由 Haspin 完成，使 Aurora B 在有丝分裂中定位于着丝粒。
Science. 2010 Oct 8;330(6001):231-5. doi: 10.1126/science.1189435. Epub 2010 Aug 12.
Zotero 插件