Key Laboratory of Pesticide and Chemical Biology of Ministry of Education, College of Chemistry, Central China Normal University, Wuhan 430079, PR China.
Nanoscale. 2012 Aug 7;4(15):4674-9. doi: 10.1039/c2nr30976a. Epub 2012 Jun 26.
We demonstrate a facile procedure to efficiently prepare Prussian blue nanocubes/reduced graphene oxide (PBNCs/rGO) nanocomposite by directly mixing Fe(3+) and Fe(CN)(6) in the presence of GO in polyethyleneimine aqueous solution, resulting in a novel acetylcholinesterase (AChE) biosensor for detection of organophosphorus pesticides (OPs). The obtained nanocomposite was characterized by X-ray photoelectron spectroscopy (XPS), transmission electron microscopy (TEM) and energy-dispersive X-ray (EDX) microanalysis. It was clearly observed that the nanosheet has been decorated with cubic PB nanoparticles and nearly all the nanoparticles are distributed uniformly only on the surface of the reduced GO. No isolated PB nanoparticles were observed, indicating the strong interaction between PB nanocubes and the reduced GO and the formation of PBNCs/rGO nanocomposite. The obtained PBNCs/rGO based AChE biosensor make the peak potential shift negatively to 220 mV. The over-potential decreases ∼460 mV compared to that on a bare electrode, suggesting that PBNCs/rGO has a high electrocatalytic activity towards the oxidation of thiocholine. The AChE biosensor shows rapid response and high sensitivity for detection of monocrotophos with a linear range from 1.0 to 600 ng mL(-1) and a detection limit of 0.1 ng mL(-1). These results suggest that the PBNCs/rGO hybrids nanocomposite exhibited high electrocatalytic activity towards the oxidation of thiocholine, which lead to the sensitive detection of OP pesticides.
我们展示了一种简便的方法，通过在 GO 存在下将 Fe(3+)和Fe(CN)(6)直接混合在聚乙烯亚胺水溶液中，有效地制备普鲁士蓝纳米立方/还原氧化石墨烯(PBNCs/rGO)纳米复合材料，从而得到一种用于检测有机磷农药(OPs)的新型乙酰胆碱酯酶(AChE)生物传感器。所得到的纳米复合材料通过 X 射线光电子能谱(XPS)、透射电子显微镜(TEM)和能量色散 X 射线(EDX)微分析进行了表征。可以清楚地观察到纳米片已被立方 PB 纳米粒子修饰，并且几乎所有的纳米粒子仅均匀分布在还原 GO 的表面上。未观察到孤立的 PB 纳米粒子，表明 PB 纳米立方与还原 GO 之间具有强相互作用，并且形成了 PBNCs/rGO 纳米复合材料。基于所获得的 PBNCs/rGO 的 AChE 生物传感器使峰电势负移至 220 mV。与裸电极相比，过电势降低了约 460 mV，表明 PBNCs/rGO 对硫代胆碱的氧化具有高电催化活性。AChE 生物传感器对氧乐果的检测具有快速的响应和高灵敏度，线性范围为 1.0 至 600 ng mL(-1)，检测限为 0.1 ng mL(-1)。这些结果表明，PBNCs/rGO 杂化纳米复合材料对硫代胆碱的氧化表现出高电催化活性，从而实现了对 OP 农药的敏感检测。
