循环 microRNA 是胆道闭锁的生物标志物。
Circulating microRNA is a biomarker of biliary atresia.
机构信息
Division of Gastroenterology and Nutrition, Department of Pediatrics, University of Pennsylvania School of Medicine, Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
出版信息
J Pediatr Gastroenterol Nutr. 2012 Oct;55(4):366-9. doi: 10.1097/MPG.0b013e318264e648.
Abstract
OBJECTIVE
The lack of reliable noninvasive diagnostic biomarkers of biliary atresia (BA) results in delayed diagnosis and worsened patient outcome. Circulating microRNAs (miRNAs) are a new class of noninvasive biomarkers with encouraging diagnostic utility.
METHODS
We examined the ability of serum miRNAs to distinguish BA from other forms of neonatal hyperbilirubinemia. BA-specific serum miRNAs were identified using a microfluidic array platform and validated in a larger, independent sample set.
RESULTS
The miR-200b/429 cluster was significantly increased in the sera of patients with BA relative to infants with non-BA cholestatic disorders.
CONCLUSIONS
Circulating levels of the miR-200b/429 cluster are elevated in infants with BA and have promising diagnostic clinical performance.
摘要
目的
胆道闭锁（BA）缺乏可靠的无创性诊断生物标志物，导致诊断延迟和患者预后恶化。循环 microRNAs（miRNAs）是一类新的无创性生物标志物，具有令人鼓舞的诊断效用。
方法
我们研究了血清 miRNAs 区分 BA 与其他新生儿高胆红素血症的能力。使用微流控芯片平台鉴定 BA 特异性血清 miRNAs，并在更大的独立样本集中进行验证。
结果
与非 BA 胆汁淤积性疾病的婴儿相比，BA 患者血清中的 miR-200b/429 簇显著升高。
结论
BA 婴儿循环 miR-200b/429 簇水平升高，具有有前景的诊断临床性能。
相似文献
1
Circulating microRNA is a biomarker of biliary atresia.循环 microRNA 是胆道闭锁的生物标志物。
J Pediatr Gastroenterol Nutr. 2012 Oct;55(4):366-9. doi: 10.1097/MPG.0b013e318264e648.
2
Liquid chromatography-mass spectroscopy in the diagnosis of biliary atresia in children with hyperbilirubinemia.液相色谱-质谱联用技术在高胆红素血症患儿胆道闭锁诊断中的应用
J Surg Res. 2018 Aug;228:228-237. doi: 10.1016/j.jss.2018.03.021. Epub 2018 Apr 11.
3
Serum markers may distinguish biliary atresia from other forms of neonatal cholestasis.血清标志物可区分胆汁淤积症与其他形式的新生儿胆汁淤积。
J Pediatr Gastroenterol Nutr. 2010 Apr;50(4):411-6. doi: 10.1097/MPG.0b013e3181cb42ee.
4
Serum microRNA microarray analysis identifies miR-4429 and miR-4689 are potential diagnostic biomarkers for biliary atresia.血清微小RNA微阵列分析表明，miR-4429和miR-4689是胆道闭锁潜在的诊断生物标志物。
Sci Rep. 2016 Feb 16;6:21084. doi: 10.1038/srep21084.
5
Identification of Circulating MicroRNAs in Biliary Atresia by Next-Generation Sequencing.通过新一代测序技术鉴定胆道闭锁患者循环中的微小RNA
J Pediatr Gastroenterol Nutr. 2016 Nov;63(5):518-523. doi: 10.1097/MPG.0000000000001194.
6
Serum matrix metalloproteinase-7 for discriminating biliary atresia: a diagnostic accuracy and validation study.血清基质金属蛋白酶-7鉴别先天性胆道闭锁：一项诊断准确性和验证研究。
J Transl Med. 2024 Jul 8;22(1):636. doi: 10.1186/s12967-024-05442-x.
7
Clinical Assessment of Differential Diagnostic Methods in Infants with Cholestasis due to Biliary Atresia or Non-Biliary Atresia.临床评估胆道闭锁或非胆道闭锁婴儿胆汁淤积的鉴别诊断方法。
Curr Med Sci. 2018 Feb;38(1):137-143. doi: 10.1007/s11596-018-1857-6. Epub 2018 Mar 15.
8
Biliary atresia and cholestasis plasma non-targeted metabolomics unravels perturbed metabolic pathways and unveils a diagnostic model for biliary atresia.胆道闭锁和胆汁淤积症的非靶向代谢组学揭示了紊乱的代谢途径，并为胆道闭锁症建立了一个诊断模型。
Sci Rep. 2024 Jul 9;14(1):15796. doi: 10.1038/s41598-024-66893-2.
9
Development and Validation of Novel Diagnostic Models for Biliary Atresia in a Large Cohort of Chinese Patients.中文译文：在一个大型中国患者队列中开发和验证新型胆道闭锁诊断模型。
EBioMedicine. 2018 Aug;34:223-230. doi: 10.1016/j.ebiom.2018.07.025. Epub 2018 Aug 1.
10
Identification of HSP90 as potential biomarker of biliary atresia using two-dimensional electrophoresis and mass spectrometry.采用二维电泳和质谱联用技术鉴定 HSP90 作为胆道闭锁的潜在生物标志物。
PLoS One. 2013 Jul 11;8(7):e68602. doi: 10.1371/journal.pone.0068602. Print 2013.
引用本文的文献
1
Emerging Biomarker Potential of Extracellular Vesicle-Enclosed MicroRNAs for Liver Fibrosis Detection.细胞外囊泡包裹的微小RNA在肝纤维化检测中的新兴生物标志物潜力
Cells. 2025 Jul 4;14(13):1025. doi: 10.3390/cells14131025.
2
Genetic background and biliary atresia.遗传背景与胆道闭锁
World J Pediatr Surg. 2025 Jun 6;8(3):e001023. doi: 10.1136/wjps-2025-001023. eCollection 2025.
3
Biliary atresia and cholestasis plasma non-targeted metabolomics unravels perturbed metabolic pathways and unveils a diagnostic model for biliary atresia.胆道闭锁和胆汁淤积症的非靶向代谢组学揭示了紊乱的代谢途径，并为胆道闭锁症建立了一个诊断模型。
Sci Rep. 2024 Jul 9;14(1):15796. doi: 10.1038/s41598-024-66893-2.
4
Comparison of Mir122 expression in children with biliary atresia and healthy group.胆道闭锁患儿与健康组中Mir122表达的比较。
Mol Biol Res Commun. 2024;13(3):147-154. doi: 10.22099/mbrc.2024.49649.1950.
5
Progress in Biomarkers Related to Biliary Atresia.与胆道闭锁相关的生物标志物研究进展
J Clin Transl Hepatol. 2024 Mar 28;12(3):305-315. doi: 10.14218/JCTH.2023.00260. Epub 2024 Jan 30.
6
Epigenetics of Inflammatory Bowel Diseases.炎症性肠病的表观遗传学。
Turk J Gastroenterol. 2023 May;34(5):437-448. doi: 10.5152/tjg.2023.22515.
7
Biliary Atresia in Children: Update on Disease Mechanism, Therapies, and Patient Outcomes.儿童先天性胆道闭锁：疾病机制、治疗方法和患者预后的最新研究进展。
Clin Liver Dis. 2022 Aug;26(3):341-354. doi: 10.1016/j.cld.2022.03.001. Epub 2022 Jun 25.
8
Biomarkers for the diagnosis and post-Kasai portoenterostomy prognosis of biliary atresia: a systematic review and meta-analysis.用于胆道闭锁诊断及葛西肝门空肠吻合术后预后评估的生物标志物：一项系统综述与荟萃分析
Sci Rep. 2021 Jun 3;11(1):11692. doi: 10.1038/s41598-021-91072-y.
9
Biliary atresia: pathology, etiology and pathogenesis.胆道闭锁：病理学、病因学与发病机制
Future Sci OA. 2020 Mar 17;6(5):FSO466. doi: 10.2144/fsoa-2019-0153.
10
RNA-seq reveals outcome-specific gene expression of MMP7 and PCK1 in biliary atresia.RNA 测序揭示了胆道闭锁中 MMP7 和 PCK1 的预后特异性表达。
Mol Biol Rep. 2019 Oct;46(5):5123-5130. doi: 10.1007/s11033-019-04969-3. Epub 2019 Jul 24.
本文引用的文献
1
MicroRNA profiling identifies miR-29 as a regulator of disease-associated pathways in experimental biliary atresia.microRNA 谱分析鉴定 miR-29 作为实验性胆道闭锁中疾病相关途径的调节剂。
J Pediatr Gastroenterol Nutr. 2012 Feb;54(2):186-92. doi: 10.1097/MPG.0b013e318244148b.
2
Novel diagnostic value of circulating miR-18a in plasma of patients with pancreatic cancer.循环 miR-18a 在胰腺癌患者血浆中的诊断新价值。
Br J Cancer. 2011 Nov 22;105(11):1733-40. doi: 10.1038/bjc.2011.453. Epub 2011 Nov 1.
3
Serum IL-6 and IL-8 in infants with biliary atresia in comparison to intrahepatic cholestasis.与肝内胆汁淤积症相比，胆道闭锁婴儿的血清白细胞介素-6和白细胞介素-8水平
Trop Gastroenterol. 2011 Jan-Mar;32(1):50-5.
4
Circulating microRNAs let-7a and miR-16 predict progression-free survival and overall survival in patients with myelodysplastic syndrome.循环 microRNAs let-7a 和 miR-16 可预测骨髓增生异常综合征患者的无进展生存和总生存。
Blood. 2011 Jul 14;118(2):413-5. doi: 10.1182/blood-2011-01-330704. Epub 2011 May 20.
5
Circulating microRNA is a biomarker of pediatric Crohn disease.循环 microRNA 是小儿克罗恩病的生物标志物。
J Pediatr Gastroenterol Nutr. 2011 Jul;53(1):26-33. doi: 10.1097/MPG.0b013e31822200cc.
6
α-enolase autoantibodies cross-reactive to viral proteins in a mouse model of biliary atresia.α-烯醇化酶自身抗体与胆道闭锁小鼠模型中病毒蛋白的交叉反应性。
Gastroenterology. 2010 Nov;139(5):1753-61. doi: 10.1053/j.gastro.2010.07.042. Epub 2010 Jul 23.
7
Detection of differentially expressed microRNAs in serum of pancreatic ductal adenocarcinoma patients: miR-196a could be a potential marker for poor prognosis.检测胰腺导管腺癌患者血清中差异表达的 microRNAs：miR-196a 可能是预后不良的潜在标志物。
Dig Dis Sci. 2011 Feb;56(2):602-9. doi: 10.1007/s10620-010-1285-3. Epub 2010 Jul 8.
8
Vesicle-related microRNAs in plasma of nonsmall cell lung cancer patients and correlation with survival.非小细胞肺癌患者血浆中与囊泡相关的 microRNAs 及其与生存的相关性。
Eur Respir J. 2011 Mar;37(3):617-23. doi: 10.1183/09031936.00029610. Epub 2010 Jul 1.
9
Serum markers may distinguish biliary atresia from other forms of neonatal cholestasis.血清标志物可区分胆汁淤积症与其他形式的新生儿胆汁淤积。
J Pediatr Gastroenterol Nutr. 2010 Apr;50(4):411-6. doi: 10.1097/MPG.0b013e3181cb42ee.
10
Plasma MicroRNAs as sensitive and specific biomarkers of tissue injury.血浆微小RNA作为组织损伤的敏感且特异的生物标志物。
Clin Chem. 2009 Nov;55(11):1977-83. doi: 10.1373/clinchem.2009.131797. Epub 2009 Sep 10.
Zotero 插件