Division of Gastroenterology and Nutrition, Department of Pediatrics, University of Pennsylvania School of Medicine, Children's Hospital of Philadelphia Research Institute, Philadelphia, PA 19104, USA.
J Pediatr Gastroenterol Nutr. 2012 Oct;55(4):366-9. doi: 10.1097/MPG.0b013e318264e648.
The lack of reliable noninvasive diagnostic biomarkers of biliary atresia (BA) results in delayed diagnosis and worsened patient outcome. Circulating microRNAs (miRNAs) are a new class of noninvasive biomarkers with encouraging diagnostic utility.
We examined the ability of serum miRNAs to distinguish BA from other forms of neonatal hyperbilirubinemia. BA-specific serum miRNAs were identified using a microfluidic array platform and validated in a larger, independent sample set.
The miR-200b/429 cluster was significantly increased in the sera of patients with BA relative to infants with non-BA cholestatic disorders.
Circulating levels of the miR-200b/429 cluster are elevated in infants with BA and have promising diagnostic clinical performance.
胆道闭锁（BA）缺乏可靠的无创性诊断生物标志物，导致诊断延迟和患者预后恶化。循环 microRNAs（miRNAs）是一类新的无创性生物标志物，具有令人鼓舞的诊断效用。
我们研究了血清 miRNAs 区分 BA 与其他新生儿高胆红素血症的能力。使用微流控芯片平台鉴定 BA 特异性血清 miRNAs，并在更大的独立样本集中进行验证。
与非 BA 胆汁淤积性疾病的婴儿相比，BA 患者血清中的 miR-200b/429 簇显著升高。
BA 婴儿循环 miR-200b/429 簇水平升高，具有有前景的诊断临床性能。
