Takizawa K, Yokoo I, Shima Y, Ozaki I, Sato M, Iguchi T, Takeda Y
Department of Obstetrics and Gynecology, Tokyo Women's Medical College.
Nihon Sanka Fujinka Gakkai Zasshi. 1990 Nov;42(11):1484-8.
Small oocytes in 6-week-old C57BL/6 mice were destroyed in a dose-dependent fashion following intraperitoneal infusion of CDDP. ED65, the effective dose of which theoretically produced 65% destruction of small oocytes according to Probit analysis, was determined as 0.2mg/mouse. In this study, the oocyte toxicity induced with intraperitoneal treatment with CDDP (0.2mg) was offset by subcutaneous treatment with STS (40mg, 400 x molar ratio compared to that of CDDP). STS was subcutaneously injected into the back skin of mice at 60, 30, 15 minutes before, at the same time or at 15, 30, 60 minutes after the CDDP injection. STS markedly diminished the oocyte toxicity and reduced oocyte destruction about 45% when it was injected at 30, 15 minutes before or at the same time as CDDP infusion. On the other hand STS failed to reduce oocyte destruction when it was injected 15, 30 or 60 minutes later. STS might neutralize CDDP in the circulating blood to decrease the amount of active CDDP, but STS might not have any effect on CDDP once it is incorporated and conjugated with the DNA in the oocytes.
在6周龄的C57BL / 6小鼠中，腹腔注射顺铂后，小卵母细胞以剂量依赖的方式被破坏。根据概率分析，理论上能产生65%小卵母细胞破坏的有效剂量ED65被确定为0.2mg/小鼠。在本研究中，腹腔注射顺铂（0.2mg）诱导的卵母细胞毒性可通过皮下注射STS（40mg，与顺铂的摩尔比为400倍）来抵消。在顺铂注射前60、30、15分钟、同时或注射后15、30、60分钟，将STS皮下注射到小鼠背部皮肤。当在顺铂输注前30、15分钟或同时注射STS时，它能显著降低卵母细胞毒性，并使卵母细胞破坏减少约45%。另一方面，当在顺铂注射后15、30或60分钟注射STS时，它未能减少卵母细胞破坏。STS可能会中和循环血液中的顺铂，以减少活性顺铂的量，但一旦顺铂与卵母细胞中的DNA结合并共轭，STS可能对其没有任何影响。
