Department of Oral Pathology, School of Dentistry and Institute of Oral Bioscience, BK21 Project, Chonbuk National University, Jeonju, Republic of Korea.
Head Neck. 2013 Jul;35(7):992-8. doi: 10.1002/hed.23072. Epub 2012 Jun 25.
The aims of this study were to evaluate the apoptotic activities and molecular mechanisms of methanol extracts of Dianthus chinensis (MEDC) and Acalypha australis L. (MEAL) in human oral cancer cells.
The apoptotic effects and related molecular mechanisms of MEDC and MEAL on oral cancer cells were evaluated using MTS assay, DAPI staining, immunostaining, Western blotting, and reverse transcriptase-polymerase chain reaction.
Sp1 was overexpressed in oral tumor tissues compared with normal oral mucosa. Downregulation of Sp1 inhibited the growth of SCC-15 and YD-15 oral cancer cells. MEDC and MEAL inhibited cell growth and induced apoptosis in both cell lines by decreasing the expression of Sp1. In addition, treatment of cells with MEDC and MEAL decreased Mcl-1 expression, which is a downstream target of Sp1.
Our results indicate that MEDC and MEAL are bioactive natural products that can potentially induce apoptosis of tumor cells that overexpress the Sp1 protein.
本研究旨在评估中国石竹甲醇提取物（MEDC）和苘麻甲醇提取物（MEAL）对人口腔癌细胞凋亡活性和分子机制的影响。
采用 MTS 法、DAPI 染色、免疫染色、Western blot 和逆转录-聚合酶链反应评估 MEDC 和 MEAL 对口腔癌细胞的凋亡作用及相关分子机制。
与正常口腔黏膜相比，口腔肿瘤组织中 Sp1 过表达。下调 Sp1 抑制 SCC-15 和 YD-15 口腔癌细胞的生长。MEDC 和 MEAL 通过降低 Sp1 的表达抑制两种细胞系的细胞生长并诱导细胞凋亡。此外，用 MEDC 和 MEAL 处理细胞可降低 Sp1 的下游靶标 Mcl-1 的表达。
我们的研究结果表明，MEDC 和 MEAL 是具有生物活性的天然产物，可潜在诱导 Sp1 蛋白过表达的肿瘤细胞凋亡。
