Departamento de Microbiología, Facultad de Biología, Universidad de Barcelona, Diagonal 645, 08071 Barcelona, Spain.
J Biol Chem. 2012 Aug 10;287(33):27851-62. doi: 10.1074/jbc.M112.376525. Epub 2012 Jun 25.
Polar and lateral flagellin proteins from Aeromonas hydrophila strain AH-3 (serotype O34) were found to be glycosylated with different carbohydrate moieties. The lateral flagellin was modified at three sites in O-linkage, with a single monosaccharide of 376 Da, which we show to be a pseudaminic acid derivative. The polar flagellin was modified with a heterogeneous glycan, comprised of a heptasaccharide, linked through the same 376-Da sugar to the protein backbone, also in O-linkage. In-frame deletion mutants of pseudaminic acid biosynthetic genes pseB and pseF homologues resulted in abolition of polar and lateral flagellar formation by posttranscriptional regulation of the flagellins, which was restored by complementation with wild type pseB or F homologues or Campylobacter pseB and F.
从气单胞菌 AH-3（血清型 O34）中发现的极地和侧向鞭毛蛋白与不同的碳水化合物部分发生了糖基化。侧向鞭毛蛋白在 O 连接中修饰了三个部位，带有一个 376Da 的单糖，我们证明它是假氨基糖衍生物。极地鞭毛蛋白与异质聚糖结合，该聚糖由七糖组成，通过相同的 376-Da 糖与蛋白骨架连接，也呈 O 连接。假氨基糖生物合成基因 pseB 和 pseF 同源物的框内缺失突变导致鞭毛蛋白的转录后调控而消除了极地和侧向鞭毛的形成，这可以通过用野生型 pseB 或 F 同源物或弯曲杆菌 pseB 和 F 进行互补来恢复。
