开发并鉴定了九对高度多态的侧柏（柏科）微卫星引物。
Development and characterization of nine highly polymorphic microsatellite primers for Platycladus orientalis (Cupressaceae).
机构信息
State Key Laboratory of Earth Surface Processes and Resource Ecology, College of Life Sciences of Beijing Normal University, Beijing, People's Republic of China.
出版信息
Am J Bot. 2012 Jul;99(7):e280-2. doi: 10.3732/ajb.1100583. Epub 2012 Jun 25.
PREMISE OF THE STUDY
Microsatellite primers were developed and characterized for the long-lived tree species Platycladus orientalis for further investigation of its population characteristics.
METHODS AND RESULTS
Nine polymorphic microsatellites were identified for P. orientalis. The number of alleles per locus ranged from four to 22 (average = 11) for 94 individual specimens examined from four populations. The observed and expected heterozygosities ranged from 0.208 to 1.000 and 0.555 to 0.931, respectively.
CONCLUSIONS
These nine markers can be used in future studies examining population genetics and the reproductive biology of P. orientalis, thereby revealing the importance of old-growth populations for the retention of genetic diversity, and providing useful insights into the maintenance of the potential genetic resource of this species.
研究前提
为进一步研究其种群特征，开发并鉴定了长寿命树种侧柏的微卫星引物。
方法与结果
鉴定出 9 个多态性微卫星位点，对来自四个种群的 94 个个体样本进行检测，每个位点的等位基因数范围为 4 到 22（平均值=11）。观测杂合度和期望杂合度分别为 0.208 到 1.000 和 0.555 到 0.931。
结论
这 9 个标记可以用于未来研究，研究侧柏的种群遗传学和生殖生物学，从而揭示老龄种群对遗传多样性保持的重要性，并为该物种潜在遗传资源的维持提供有用的见解。
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