Institute of Wood Technology, Akita Prefectural University, Noshiro, Japan.
Am J Bot. 2012 Aug;99(8):e317-9. doi: 10.3732/ajb.1200010. Epub 2012 Aug 1.
Microsatellite markers were developed for the endemic Japanese species Thujopsis dolabrata var. hondai for studies on forest ecology and tree breeding.
We characterized 10 dinucleotide microsatellite loci by screening primers developed using a simple sequence repeat-enriched library. The number of alleles per locus ranged from eight to 44 with an average of 20.3. The observed and expected heterozygosities ranged from 0.326 to 0.854 and from 0.670 to 0.976, respectively.
The development of these markers can be used to assess useful genetic information for ecological studies and tree breeding in T. dolabrata var. hondai.
开发出微卫星标记，用于研究本地日本物种池杉 var. hondai 的森林生态学和树木育种。
我们通过筛选使用简单重复序列富集文库开发的引物，对 10 个二核苷酸微卫星位点进行了特征描述。每个位点的等位基因数范围从 8 到 44，平均为 20.3。观察到的和预期的杂合度分别为 0.326 至 0.854 和 0.670 至 0.976。
这些标记的开发可用于评估池杉 var. hondai 生态研究和树木育种的有用遗传信息。
