Zhao Zhao-Xia, Lu Jian-Hua, Mei Zhi-Qin, Pan Shi-Yang, Chen Dan, Liu Yu-Zhen, Xia Wen-Ying, Dai Er-Hei
Department of Laboratory Medicine, Fifth Hospital of Shijiazhuang, Shijiazhuang 050021, China.
Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Dec;25(6):483-5.
To determine the circular DNA level of patients with hand foot and mouth disease (HFMD) and evaluate its potential clinical value.
Venous blood in 30 healthy children and 78 patients with HFMD within 3 days of onset of illness and convalescent period was collected. The level of plasma circular DNA was detected by duplex real-time polymerase chain reaction assay. Blood sugar, high-sensitive CRP(hs-CRP) and leucocyte were also detected.
The level of circular DNA in control group was (6.57 +/- 4.67) ng/ml. The level of circular DNA in ordinary and severe HFMD patients was (11.51 +/- 7.75) ng/ml and (20.59 +/- 10.67) ng/ml before treatment, respectively. The levels of circular DNA in ordinary and severe HFMD patients were significantly higher than that in control group (P = 0.021; 0.000); the level of circular DNA in severe HFMD patients was significantly higher than that in ordinary HFMD patients (P = 0.011). The level of circular DNA in severe HFMD patients after treatment were significantly lower than that before treatment (P = 0.033). The level of circular DNA before treatment and after treatment in ordinary HFMD patients had no significant difference. The levels of blood sugar and hs-CRP in severe HFMD patients were higher than those in ordinary before treatment (P = 0.045; 0.011). The levels of blood sugar and hs-CRP before treatment and after treatment in ordinary HFMD patients had no significant change. There was significantly positive correlation between the level of circular DNA and that of hs-CRP in HFMD patient (P = 0.021), but there was no correlation between the level of circular DNA and that of blood sugar and leucocyte.
The level of circular DNA not only become an early identification marker of severe HFMD patients, but also become monitoring marker of effect of treatment.
测定手足口病（HFMD）患者的环状DNA水平，并评估其潜在的临床价值。
收集30例健康儿童以及78例手足口病患者发病3天内及恢复期的静脉血。采用双重实时聚合酶链反应法检测血浆环状DNA水平。同时检测血糖、高敏C反应蛋白（hs-CRP）和白细胞。
对照组环状DNA水平为（6.57±4.67）ng/ml。普通型和重型手足口病患者治疗前环状DNA水平分别为（11.51±7.75）ng/ml和（20.59±10.67）ng/ml。普通型和重型手足口病患者的环状DNA水平均显著高于对照组（P = 0.021；0.000）；重型手足口病患者的环状DNA水平显著高于普通型手足口病患者（P = 0.011）。重型手足口病患者治疗后的环状DNA水平显著低于治疗前（P = 0.033）。普通型手足口病患者治疗前后的环状DNA水平无显著差异。重型手足口病患者治疗前的血糖和hs-CRP水平高于普通型（P = 0.045；0.011）。普通型手足口病患者治疗前后的血糖和hs-CRP水平无显著变化。手足口病患者环状DNA水平与hs-CRP水平呈显著正相关（P = 0.021），但环状DNA水平与血糖及白细胞水平无相关性。
环状DNA水平不仅可成为重型手足口病患者的早期识别标志物，还可成为治疗效果的监测标志物。
