[乙肝表面抗原与乙肝表面抗体共存血清中乙肝表面抗体确证试验的建立]

[Establishment of confirmatory test for HBsAb in serum of coexistence of hepatitis B surface antigen and antibodies to HBsAg].

作者信息

Liu Jia, Chen Lin, Xu Jju, Guo Jing-Xia, Song Yong-Ji, Zhao Jing, Liu Ai-Xia, Yang Li-Hua, Li Bo-An, Mao Yuan-Li

机构信息

Center of Clinical Laboratory, The No. 302 Hospital of The PLA, Beijing 100039, China.

出版信息

Zhonghua Shi Yan He Lin Chuang Bing Du Xue Za Zhi. 2011 Dec;25(6):492-4.

PMID:22734246

Abstract

OBJECTIVE

Establish a kind of confirmation method based on ELISA, and use to verify authenticity of HBsAb + in HBsAg + HBsAb + serum, pick and get rid of the false masculine gender the result, and avoid the mistake diagnosis.

METHOD

Collect 60 pieces of serum whose thick degree of HBsAg at 1000 COI above tested by ECLIA as confirm serum, mixed the confirm serum of different dilution with HBsAb positive serum to screen and verify best thick degree of HBsAg. Collected 40 pieces of HBsAg + HBsAb + serum, ELISA tested the descend rate of HBsAb COI after neutralized with confirm serum in order to confirm authenticity of HBsAb + in pieces of HBsAg + HBsAb + serum.

RESULT

When thick degree of HBsAg is 2000 COI, the performance of neutralization to HBsAb is best. The ELISA confirmatory test is fully consistent with the ECLIA method with true positive of 37 pieces of HBsAg + HBsAb + serum while false-positive of 3 pieces of serum.

CONCLUSION

The ELISA confirm method is a simple, accurate and low cost initial validation method.

摘要

目的

建立一种基于酶联免疫吸附测定（ELISA）的确认方法，用于验证HBsAg+HbsAb+血清中HbsAb+的真实性，剔除假阳性结果，避免误诊。

方法

收集60份经电化学发光免疫分析（ECLIA）检测HBsAg浓度在1000 COI以上的血清作为确认血清，将不同稀释度的确认血清与HbsAb阳性血清混合，筛选并验证最佳HBsAg浓度。收集40份HBsAg+HbsAb+血清，用确认血清中和后，通过ELISA检测HbsAb COI的下降率，以确认各份HBsAg+HbsAb+血清中HbsAb+的真实性。