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单纯疱疹病毒附着的研究。胰蛋白酶处理的效果。

Studies on the attachment of herpes simplex virus. Effect of trypsin treatment.

作者信息

Blomberg J

出版信息

Arch Virol. 1979;61(3):201-6. doi: 10.1007/BF01318054.

DOI:10.1007/BF01318054
PMID:227343
Abstract

The adsorption of herpes simplex virus (HSV) to monolayers of trypsinized and mock-treated GMK-AH1 cells was studied by means of a microplaque technique. Plaque formation was nearly abolished for c:a 20 minutes and full sensitivity regained at 6--8 hours post trypsinization. The return of sensitivity to HSV was inhibited by cycloheximide and incubation at 4 degrees C. The sum of PFU in the supernatant and PFU successe modifying agents were without effect on the adsorption of HSV.

摘要

采用微量空斑技术研究了单纯疱疹病毒(HSV)对经胰蛋白酶处理和模拟处理的GMK-AH1细胞单层的吸附情况。空斑形成在约20分钟内几乎被消除,胰蛋白酶处理后6 - 8小时恢复完全敏感性。环己酰亚胺和在4℃下孵育可抑制对HSV敏感性的恢复。上清液中的空斑形成单位(PFU)总和以及成功修饰剂对HSV的吸附没有影响。

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本文引用的文献

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