Studies on the attachment of herpes simplex virus. Effect of trypsin treatment.

The adsorption of herpes simplex virus (HSV) to monolayers of trypsinized and mock-treated GMK-AH1 cells was studied by means of a microplaque technique. Plaque formation was nearly abolished for c:a 20 minutes and full sensitivity regained at 6--8 hours post trypsinization. The return of sensitivity to HSV was inhibited by cycloheximide and incubation at 4 degrees C. The sum of PFU in the supernatant and PFU successe modifying agents were without effect on the adsorption of HSV.