Ando T, Arai H
J Gen Virol. 1980 Jun;48(Pt 2):319-28. doi: 10.1099/0022-1317-48-2-319.
The nature of the refractoriness of C6 glioblastoma cells to herpes simplex virus type I (HSV-I) infection has been studied. The cells were restricted in susceptibility to HSV-I since only a small proportion of the cells could be infected by HSV-I and the virus yield per cell was low. The susceptibility to infection was increased by treating the cells with trypsin-EDTA prior to infection. The cells so treated recovered resistance to the virus when incubated at 37 degrees C, their resistance being restored to the initial level in 2 days. This restoration was inhibited by addition of cycloheximide or puromycin. Trypsin-EDTA treatment of C6 cells increased the efficiency of adsorption of HSV-I and the formation of stable cell-virus complexes from which the virus could not be dissociated by heparin.
已对C6胶质母细胞瘤细胞对I型单纯疱疹病毒（HSV-I）感染的难治性本质进行了研究。这些细胞对HSV-I的易感性受到限制，因为只有一小部分细胞能够被HSV-I感染，且每个细胞产生的病毒量较低。在感染前用胰蛋白酶-乙二胺四乙酸（trypsin-EDTA）处理细胞可增加其对感染的易感性。如此处理过的细胞在37℃孵育时会恢复对病毒的抗性，其抗性在2天内恢复到初始水平。添加环己酰亚胺或嘌呤霉素可抑制这种恢复。用胰蛋白酶-EDTA处理C6细胞可提高HSV-I的吸附效率以及形成稳定的细胞-病毒复合物，而肝素无法从该复合物中解离出病毒。
