[胡椒的生物活性成分研究]
[Study on the bioactive constituents of Piper wallichii].
作者信息
Zhao Guo-Wei, Xia Wen, Chen Ping, Han En-Ji, Xiang Lan
机构信息
School of Pharmacy, Shandong University, Jinan 250012, China.
出版信息
Zhong Yao Cai. 2012 Jan;35(1):53-6.
OBJECTIVE
To investigate the bioactive constituents in the stem of Piper wallichii.
METHODS
Compounds were separated by column chromatography of silica gel, ODS-A and Sephadex LH-20. Their structures were elucidated based on spectral analysis. DPPH scavenging activity and AchE inhibitory activity were tested.
RESULTS
10 compounds were isolated and their structures were identified as 3,4-methylenedioxy-benzoic acid (1), vanillic acid (2), benzoic acid (3), N-p-coumaroyltyramine (4), futoenone (5), futoquinol (6), isofutoquinol A (7), 4-hydroxy-3,5-dimethoxy-benzoic acid (8), futoamide (9), dihydropiperlonguminine (10).
CONCLUSION
Compounds 1-6 are isolated from P. wallichii for the first time. Vanillic acid (2) and 4-hydroxy-3,5-dimethoxy-benzoic acid (8) show scavenging activity against DPPH radical with ED50 at 224.33 microg/mL and 11.44 microg/mL, respectively. No compound shows inhibition activity against AchE.
目的
研究毛蒌茎中的生物活性成分。
方法
采用硅胶柱色谱、ODS - A柱色谱和葡聚糖凝胶LH - 20柱色谱分离化合物。通过光谱分析确定其结构。测试了DPPH清除活性和乙酰胆碱酯酶抑制活性。
结果
分离得到10个化合物，其结构分别鉴定为3,4 - 亚甲二氧基苯甲酸（1）、香草酸（2）、苯甲酸（3）、N - 对香豆酰酪胺（4）、荜茇酮（5）、荜茇喹醇（6）、异荜茇喹醇A（7）、4 - 羟基 - 3,5 - 二甲氧基苯甲酸（8）、荜茇酰胺（9）、二氢胡椒隆明宁（10）。
结论
化合物1 - 6首次从毛蒌中分离得到。香草酸（2）和4 - 羟基 - 3,5 - 二甲氧基苯甲酸（8）对DPPH自由基具有清除活性，其半数有效浓度（ED50）分别为224.33 μg/mL和11.44 μg/mL。没有化合物表现出对乙酰胆碱酯酶的抑制活性。
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