Departament d'Enginyeria Electrònica, Elèctrica i Automàtica, Universitat Rovira i Virgili , Avenida Països Catalans 26, 43007 Tarragona, Spain.
ACS Appl Mater Interfaces. 2012 Jul 25;4(7):3584-8. doi: 10.1021/am300648j. Epub 2012 Jul 10.
Herein, we present a smart enzymatic sensor based on nanoporous anodic alumina (NAA) and its photoluminescence (PL) in the UV-visible range. The as-produced structure of NAA is functionalized and activated in order to perform the enzyme immobilization in a controlled manner. The whole process is monitored through the PL spectrum and each stage is characterized by an exclusive barcode, which is associated with the PL oscillations. This characteristic property allows us to calculate the change in the effective optical thickness that takes place after each stage. This makes it possible to accurately detect and quantify the immobilized enzyme within the NAA structure. Finally, the NAA geometry (i.e., the pore length and its diameter) is optimized to improve the enzyme immobilization and its detection inside the pores. This enzymatic sensor can give quick and accurate measurements of enzyme levels, what is crucial in clinical enzymology to prevent and detect diseases at their primary stage.
在此，我们展示了一种基于纳米多孔阳极氧化铝 (NAA) 的智能酶传感器及其在紫外可见范围内的光致发光 (PL)。所生产的 NAA 结构经过功能化和激活，以便以可控的方式进行酶固定。整个过程通过 PL 光谱进行监测，每个阶段都具有独特的条码，与 PL 振荡相关联。这种特性使我们能够计算出每个阶段后发生的有效光厚度变化。这使得能够准确检测和定量固定在 NAA 结构内的酶。最后，优化了 NAA 几何形状（即孔长度及其直径）以改善酶在孔内的固定化及其检测。这种酶传感器可以快速准确地测量酶水平，这在临床酶学中至关重要，可在疾病的初始阶段进行预防和检测。
