Milech Nadia, Watt Paul
Telethon Institute for Child Heath Research and Centre for Child Health, University of Western Australia, Subiaco, WA, Australia.
Methods Mol Biol. 2012;899:43-60. doi: 10.1007/978-1-61779-921-1_3.
Phylomer libraries are made from random overlapping genome fragments of biodiverse bacteria and Archaea. They provide a rich source of high-affinity binders to protein interfaces, and can be used both for target-directed screening approaches and for phenotypic screens to discover new targets. Here, we describe methods used for the construction of a Phylomer library, illustrated by examples of construction in both a yeast two-hybrid vector and a phage display vector.
种系分子文库由生物多样性细菌和古细菌的随机重叠基因组片段构建而成。它们为蛋白质界面提供了丰富的高亲和力结合剂来源，可用于靶向筛选方法和表型筛选以发现新靶点。在此，我们描述了构建种系分子文库所使用的方法，并以酵母双杂交载体和噬菌体展示载体中的构建实例进行说明。
