Geisse Sabine, Voedisch Bernd
Novartis Institutes for BioMedical Research, Basel, Switzerland.
Methods Mol Biol. 2012;899:203-19. doi: 10.1007/978-1-61779-921-1_13.
The first protocols describing transient gene expression in mammalian cells for the rapid generation of recombinant proteins emerged more than 10 years ago as an alternative to the establishment of stable, often amplified clonal cell lines, and relieved somewhat the bias against mammalian cell systems as being too complicated, labor intensive, and tedious to serve as a source for tool proteins in industrial research and academia. Over the past decade, these attempts have been refined and optimized, giving rise to expression protocols applicable in every lab in dependence on available tools, equipment, and envisaged outcome. This chapter summarizes the development of transient expression technologies over the past decade up to its current status and provides an outlook into what may be the future of transient technology development.
10多年前出现了首批描述在哺乳动物细胞中进行瞬时基因表达以快速生成重组蛋白的方案，作为建立稳定的、通常经过扩增的克隆细胞系的替代方法，在一定程度上缓解了人们对哺乳动物细胞系统的偏见，即认为其过于复杂、 labor intensive且繁琐，不适用于工业研究和学术界作为工具蛋白的来源。在过去十年中，这些尝试得到了改进和优化，根据可用的工具、设备和预期结果，产生了适用于每个实验室的表达方案。本章总结了过去十年中瞬时表达技术的发展直至其当前状态，并展望了瞬时技术发展的未来可能走向。 （注：“labor intensive”直译为“劳动密集型”，放在这里语义不太通顺，可意译为“耗费人力的”等合适表述）
