Lowe Adam J, Bardliving Cameron L, Batt Carl A
Graduate Field of Microbiology, Cornell University, Ithaca, NY, USA.
Methods Mol Biol. 2012;899:265-75. doi: 10.1007/978-1-61779-921-1_17.
Endotoxin removal is critical when producing therapeutic proteins in bacterial systems. This hydrophobic compound can be removed through chromatography or filtration, but presents unique challenges dependent upon protein composition as well as production scale. Here we present a robust method for endotoxin removal at the pilot production scale using fast protein liquid chromatography and buffers specifically engineered for endotoxin removal.
在内毒素去除是在细菌系统中生产治疗性蛋白质时至关重要。这种疏水性化合物可以通过色谱法或过滤去除，但根据蛋白质组成和生产规模会带来独特的挑战。在这里，我们提出了一种在中试生产规模下使用快速蛋白质液相色谱法和专门为内毒素去除设计的缓冲液进行内毒素去除的稳健方法。
