Hirayama Chuichi, Sakata Masayo
Department of Applied Chemistry & Biochemistry, Faculty of Engineering, Kumamoto University, 2-39-1 Kurokami, Kumamoto 860-8555, Japan.
J Chromatogr B Analyt Technol Biomed Life Sci. 2002 Dec 5;781(1-2):419-32. doi: 10.1016/s1570-0232(02)00430-0.
Endotoxins, constituents of cell walls of gram-negative bacteria, are potential contaminants of the protein solutions originating from biological products. Such contaminants have to be removed from solutions used for intravenous administration, because of their potent biological activities causing pyrogenic reactions. Separation methods used for decontamination of water, such as ultrafiltration, have little effect on endotoxin levels in protein solutions. To remove endotoxin from a solution of high-molecular-mass compounds, such as proteins, the adsorption method has proven to be most effective. In this review, we first introduce endotoxin-specific properties in an aqueous solution, and then provide various methods of chromatographic separation of endotoxins from cellular products using polymer adsorbents. We also provide the design of novel endotoxin-specific polymer adsorbents.
内毒素是革兰氏阴性菌细胞壁的成分，是生物制品来源的蛋白质溶液的潜在污染物。由于这些污染物具有强大的生物活性，可引起热原反应，因此必须从用于静脉注射的溶液中去除。用于水净化的分离方法，如超滤，对蛋白质溶液中的内毒素水平影响很小。事实证明，对于从蛋白质等高分子量化合物溶液中去除内毒素，吸附法最为有效。在本综述中，我们首先介绍内毒素在水溶液中的特异性性质，然后提供使用聚合物吸附剂从细胞产物中色谱分离内毒素的各种方法。我们还提供了新型内毒素特异性聚合物吸附剂的设计。
