Department of Analytical Chemistry and Pharmaceutical Technology, Center for Pharmaceutical Research, Vrije Universiteit Brussel-VUB, Brussels, Belgium.
Electrophoresis. 2012 Jun;33(11):1613-23. doi: 10.1002/elps.201100689.
Chiral monolithic silica capillary columns were prepared by immobilization of amylose-3,5-dimethylphenylcarbamate (ADMPC) bearing a small fraction of 3-(triethoxysilyl)propyl residues through intermolecular polycondensation of the triethoxysilyl groups. The obtained columns were used for chiral separations in capillary electrochromatography (CEC). The effects of the silica monolith nature and the used chiral selector concentration on the resulting enantiomeric separations were investigated. Fifteen chiral compounds, including acidic, neutral, and basic substances were evaluated and twelve showed partial or baseline separation at some of the different conditions tested. These results demonstrated the promising applicability of ADMPC-immobilized monolithic silica columns in CEC enantioseparations, but also revealed the need for further improvements on the level of baseline separations and efficiencies.
手性整体硅胶毛细管柱通过 3-（三乙氧基硅基）丙基残基的少量 3,5-二甲基苯甲酰基纤维素（ADMPC）的分子间缩聚固定化制备。所得柱用于胶束电动色谱（CEC）中的手性分离。考察了硅胶整体性质和手性选择剂浓度对所得对映体分离的影响。评估了 15 种手性化合物，包括酸性、中性和碱性物质，其中 12 种在测试的不同条件下部分或基线分离。这些结果表明 ADMPC 固定化整体硅胶柱在手性 CE 分离中的应用具有广阔的前景，但也表明需要进一步提高基线分离度和效率。
