Chankvetadze Bezhan, Yamamoto Chiyo, Kamigaito Masami, Tanaka Nobuo, Nakanishi Kazuki, Okamoto Yoshio
Molecular Recognition and Separation Science Laboratory, School of Chemistry, Tbilisi State University, Chavchavadze Ave 1, 028 Tbilisi, Georgia.
J Chromatogr A. 2006 Mar 31;1110(1-2):46-52. doi: 10.1016/j.chroma.2006.01.076. Epub 2006 Feb 14.
Monolithic capillary columns containing native silica were modified by in situ coating with amylose tris(3,5-dimethylphenylcarbamate) and applied for enantioseparations in capillary liquid chromatography. Capillary columns were examined for 10 standard racemic compounds in order to compare the performance of monolithic silica columns with the common, 4.6mm I.D. high-performance liquid chromatographic columns packed with particulate silica. The effects of polysaccharide coating and of the linear velocity of the mobile phase on peak performance were studied. Enantioseparations with an analysis time below 1min were achieved for some chiral analytes.
含有天然二氧化硅的整体式毛细管柱通过原位涂覆直链淀粉三（3,5-二甲基苯基氨基甲酸酯）进行改性，并应用于毛细管液相色谱中的对映体分离。对毛细管柱进行了10种标准外消旋化合物的检测，以便将整体式硅胶柱的性能与常见的内径4.6mm、填充颗粒硅胶的高效液相色谱柱的性能进行比较。研究了多糖涂层和流动相线性速度对峰性能的影响。对于一些手性分析物，实现了分析时间低于1分钟的对映体分离。
