Department of Otorhinolaryngology, Ankara Training and Research Hospital, Ankara, Turkey.
Clin Exp Otorhinolaryngol. 2012 Jun;5(2):81-5. doi: 10.3342/ceo.2012.5.2.81. Epub 2012 Jun 12.
To detect the prevalences of Alloiococcus otitidis, as well as Haemophilus influenzae, Streptococcus pneumoniae, and Moraxella catarrhalis in children with chronic otitis media with effusion (OME) and to simultaneously investigate the colonization of these bacteria in the nasopharynx and palatine tonsils of these patients.
The study included 34 pediatric patients with OME, and 15 controls without OME. In the study group, A. otitidis, H. influenzae, S. pneumoniae, and M. catarrhalis were investigated in the samples obtained from middle ear effusions (MEE), nasopharyngeal swabs (NPS) and tonsillar swabs (TS), using multiplex polymerase chain reaction (PCR) and conventional culture methods. Only the samples obtained from NPS and TS were studied with the same techniques in the control group.
A. otitidis was isolated only in MEE and only with multiplex PCR method. A. otitidis, S. pneumoniae, M. catarrhalis, H. influenzae were identified in 35%, 8.8%, 8.8%, and 2.9%, respectively, in 34 MEE. A. otitidis was not isolated in NPS or TS of the study and the control groups.
The prevalence of A.otitidis is high in children with OME and A.otitidis doesn't colonize in the nasopharynx or tonsil.
检测分泌性中耳炎（OME）患儿中奇异变形菌以及流感嗜血杆菌、肺炎链球菌和卡他莫拉菌的流行情况，并同时研究这些细菌在患者鼻咽部和腭扁桃体中的定植情况。
本研究纳入了 34 名 OME 患儿和 15 名非 OME 对照患儿。在研究组中，采用多重聚合酶链反应（PCR）和常规培养方法，从中耳积液（MEE）、鼻咽拭子（NPS）和扁桃体拭子（TS）中检测奇异变形菌、流感嗜血杆菌、肺炎链球菌和卡他莫拉菌。在对照组中，仅使用相同的技术研究 NPS 和 TS 样本。
奇异变形菌仅在 MEE 中通过多重 PCR 方法分离到。34 例 MEE 中，奇异变形菌、肺炎链球菌、卡他莫拉菌和流感嗜血杆菌的检出率分别为 35%、8.8%、8.8%和 2.9%。在研究组和对照组的 NPS 或 TS 中均未分离到奇异变形菌。
OME 患儿中奇异变形菌的检出率较高，且奇异变形菌不会在鼻咽部或扁桃体定植。
