Pate Diana O, Clode Alison B, Olivry Thierry, Cullen John M, Salmon Jacklyn H, Gilger Brian C
Department of Clinical Sciences, College of Veterinary Medicine, North Carolina State University, Raleigh, NC 27607, USA.
Am J Vet Res. 2012 Jul;73(7):1067-73. doi: 10.2460/ajvr.73.7.1067.
To describe the immunopathologic characteristics of superficial stromal immune-mediated keratitis (IMMK) immunopathologically by characterizing cellular infiltrate in affected corneas of horses.
10 client-owned horses with IMMK.
Immunohistochemical staining was performed on keratectomy samples with equine antibodies against the T-cell marker CD3 and B-cell marker CD79a (10 eyes) and the T-helper cytotoxic marker CD4 and T-cell cytotoxic marker CD8 (6 eyes). Percentage of positively stained cells was scored on a scale from 0 (no cells stained) to 4 (> 75% of cells stained). Equine IgG, IgM, and IgA antibodies were used to detect corneal immunoglobulin via direct immunofluorescence (10 eyes). Serum and aqueous humor (AH) samples from 3 horses with IMMK were used to detect circulating and intraocular IgG against corneal antigens via indirect immunofluorescence on unaffected equine cornea.
Percentage scores (scale, 0 to 4) of cells expressing CD3 (median, 2.35 [range, 0.2 to 3.7]; mean ± SD, 2.36 ± 1.08) were significantly greater than scores of cells expressing CD79a (median, 0.55 [range, 0 to 1.5]; mean, 0.69 ± 0.72). All samples stained positively for CD4- and CD8-expressing cells, with no significant difference in scoring. All samples stained positively for IgG, IgM, and IgA. No serum or AH samples collected from horses with IMMK reacted with unaffected equine cornea.
Pathogenesis of superficial stromal IMMK included cell-mediated inflammation governed by both cytotoxic and helper T cells. Local immunoglobulins were present in affected corneas; however, corneal-binding immunoglobulins were not detected in the serum or AH from horses with IMMK.
通过对患马角膜中细胞浸润进行特征分析，从免疫病理学角度描述浅表基质免疫介导性角膜炎（IMMK）的免疫病理特征。
10匹患有IMMK的客户拥有的马。
对角膜切除术样本进行免疫组织化学染色，使用抗马T细胞标志物CD3和B细胞标志物CD79a的抗体（10只眼）以及辅助性T细胞细胞毒性标志物CD4和T细胞细胞毒性标志物CD8的抗体（6只眼）。对阳性染色细胞的百分比进行评分，范围从0（无细胞染色）到4（>75%的细胞染色）。使用马IgG、IgM和IgA抗体通过直接免疫荧光法检测角膜免疫球蛋白（10只眼）。采集3匹患有IMMK的马的血清和房水样本，通过对未受影响的马角膜进行间接免疫荧光法检测针对角膜抗原的循环和眼内IgG。
表达CD3的细胞百分比评分（范围0至4）（中位数，2.35[范围，0.2至3.7]；平均值±标准差，2.36±1.08）显著高于表达CD79a的细胞评分（中位数，0.55[范围，0至1.5]；平均值，0.69±0.72）。所有样本中表达CD4和CD8的细胞均呈阳性染色，评分无显著差异。所有样本中IgG、IgM和IgA均呈阳性染色。从患有IMMK的马采集的血清或房水样本与未受影响的马角膜均无反应。
浅表基质IMMK的发病机制包括由细胞毒性T细胞和辅助性T细胞共同调控的细胞介导的炎症。患病角膜中存在局部免疫球蛋白；然而，在患有IMMK的马的血清或房水中未检测到与角膜结合的免疫球蛋白。
