Woo S L, Danto M I, Ohland K J, Lee T Q, Newton P O
Department of Orthopaedic Surgery, University of Pittsburgh, PA 15261.
J Biomech Eng. 1990 Nov;112(4):426-31. doi: 10.1115/1.2891206.
Determination of the tensile stresses in ligaments and tendons during uniaxial loading depends on accurate measurement of the cross-sectional area. In this study, a laser micrometer system was employed to evaluate the cross-sectional shape and area of the medial collateral ligament (MCL) at three locations and anterior cruciate ligament (ACL). In a New Zealand White (NZW) rabbit, morphologic sections of the ligaments were made to verify the cross-sectional shape reconstructed by the laser micrometer system. The areas obtained by the laser micrometer system from ten additional NZW rabbits were compared with those obtained by two other methods commonly used to measure the cross-sectional area of ligaments and tendons: one method uses digital calipers and the other a constant pressure (0.12 MPa) area micrometer. For the MCL, the digital calipers yielded results very similar to those of the laser micrometer, but the constant pressure area micrometer yielded values 20 percent lower. The area measured at the proximal site of the MCL was 13 percent greater than the area measured at the joint line and distal line. For the ACL, the values obtained by the digital calipers and constant pressure area micrometer were 16 and 20 percent lower, respectively. Because of the irregular shape exhibited by the rabbit ACL, the digital calipers could not accurately measure the cross-sectional area. The constant pressure area micrometer yielded lower values for the cross-sectional area of both the MCL and ACL, presumably due to the applied pressure which caused changes in both the cross-sectional shape and area.
单轴加载过程中韧带和肌腱拉伸应力的测定取决于对横截面积的精确测量。在本研究中，采用激光测微仪系统评估内侧副韧带（MCL）三个位置和前交叉韧带（ACL）的横截面形状和面积。在一只新西兰白兔中制作韧带的形态学切片，以验证激光测微仪系统重建的横截面形状。将激光测微仪系统从另外十只新西兰白兔获得的面积与另外两种常用于测量韧带和肌腱横截面积的方法所获得的面积进行比较：一种方法使用数字卡尺，另一种使用恒压（0.12MPa）面积测微仪。对于MCL，数字卡尺得到的结果与激光测微仪的结果非常相似，但恒压面积测微仪得到的值低20%。在MCL近端测量的面积比在关节线和远端线测量的面积大13%。对于ACL，数字卡尺和恒压面积测微仪得到的值分别低16%和20%。由于兔ACL呈现不规则形状，数字卡尺无法准确测量横截面积。恒压面积测微仪得到的MCL和ACL横截面积值较低，可能是由于施加的压力导致横截面形状和面积都发生了变化。
