Department of Physiology, Gulhane Military Medical Academy, Ankara, Turkey.
Arch Gynecol Obstet. 2012 Nov;286(5):1201-7. doi: 10.1007/s00404-012-2435-y. Epub 2012 Jun 28.
Various studies have been performed to find out novel treatment strategies to prevent postoperative adhesion formation. Ozone therapy (OT) is shown to reduce inflammation in several pathological conditions. Therefore, we aimed to evaluate the efficacy of OT in a rat model of experimental uterine adhesion (EUA).
Thirty female Wistar rats (200-250 g) were divided into three groups: sham, EUA and EUA+OT. EUA and EUA+OT groups were subjected to the postoperative adhesion procedure by bipolar coagulation on the uterine horns and corresponding pelvic sidewall parietal peritoneum. EUA+OT group received 0.7 mg/kg daily single dose for 3 days of ozone/oxygen mixture intraperitoneally after adhesion induction. All animals were killed on the 7th day and uterine adhesions were scored. Uterine tissues and peritoneal washing fluid were harvested for all analyses.
Uterine malondialdehyde levels in the EUA group were significantly higher compared to the other groups. However, in the EUA group, uterine superoxide dismutase and glutathione peroxidase activities were lower than in other groups. Peritoneal fluid TNF-α levels were found to be significantly different for all groups (p < 0.001). Macroscopic total adhesion score was significantly higher in the EUA group compared to the other groups (p < 0.001). But, total score in the EUA+OT group was lower than in the EUA group (p = 0.006).
Medical OT prevents postoperative uterine adhesions by modulating TNF-α levels and oxidative/antioxidative status in an experimental uterine adhesion model.
为了寻找新的治疗策略来预防术后粘连形成，已经进行了各种研究。臭氧治疗（OT）在多种病理情况下显示出降低炎症的作用。因此，我们旨在评估 OT 在实验性子宫粘连（EUA）大鼠模型中的疗效。
将 30 只雌性 Wistar 大鼠（200-250 g）分为三组：假手术组、EUA 组和 EUA+OT 组。EUA 和 EUA+OT 组通过双极电凝在子宫角和相应的骨盆侧壁壁层腹膜上进行术后粘连程序。EUA+OT 组在粘连诱导后每天接受 0.7 mg/kg 的臭氧/氧气混合物单次腹腔内给药 3 天。所有动物均在第 7 天处死，并对子宫粘连进行评分。采集子宫组织和腹膜冲洗液进行所有分析。
与其他组相比，EUA 组的子宫丙二醛水平显著升高。然而，在 EUA 组中，子宫超氧化物歧化酶和谷胱甘肽过氧化物酶的活性低于其他组。各组腹膜液 TNF-α 水平差异有统计学意义（p < 0.001）。EUA 组的宏观总粘连评分明显高于其他组（p < 0.001）。但是，EUA+OT 组的总评分低于 EUA 组（p = 0.006）。
在实验性子宫粘连模型中，医用 OT 通过调节 TNF-α 水平和氧化/抗氧化状态来预防术后子宫粘连。
