Department of Chemistry and Biochemistry, California State University, Los Angeles, CA 90032-8202, USA.
Electrophoresis. 2012 Jul;33(12):1723-8. doi: 10.1002/elps.201100673.
A miniaturized capillary electrophoresis system coupled to a surface plasmon resonance (SPR) sensor on a microfluidic platform fabricated from PDMS is detailed. A previously described split-flow injection technique is first utilized to manipulate sample into the microfluidic chip, followed by separation within the fused-silica capillary and final off-capillary detection of analytes via SPR. Instead of using commercial SPR flow cells requiring relatively large detection volumes, samples of less than 1 nL volume are utilized. The interface between the CE system and SPR sensor made it possible to detect minute volumes of sample with minimal dispersion. The flow cell has the potential to be applicable to miniaturized flow-injection (FI) systems where submicroliter volumes of sample are frequently only available for analysis. The components present in solution, but not bound to the sensor surface, were also investigated. The sensitivity of the CE-SPR system was similar to that found in UV-spectrometric instruments and nonchromophoric components could also be measured.
详细介绍了一种基于 PDMS 微流控平台的小型化毛细管电泳系统，该系统与表面等离子体共振（SPR）传感器相耦合。首先利用先前描述的分流注入技术将样品操纵到微流控芯片中，然后在熔融石英毛细管内进行分离，最后通过 SPR 在毛细管外检测分析物。该系统没有使用需要相对较大检测体积的商业 SPR 流动池，而是使用了小于 1nL 体积的样品。CE 系统与 SPR 传感器之间的接口使得可以检测到最小体积的样品，且分散度最小。该流动池有可能适用于小型化流动注射（FI）系统，因为通常只有亚微升体积的样品可用于分析。还研究了溶液中存在但未与传感器表面结合的组分。CE-SPR 系统的灵敏度与在紫外光谱仪中发现的灵敏度相似，也可以测量非生色成分。
