Qian Li, Zhang Yue, Pan Xin-Yuan, Ji Ming-Chun, Gong Wei-Juan, Tian Fang
Department of Immunology, School of Medicine, Yangzhou University, Yangzhou 225001.
Oncol Lett. 2012 Feb;3(2):472-476. doi: 10.3892/ol.2011.495. Epub 2011 Nov 25.
CD8(+) T cells play critical roles in immunosurveillance by killing malignant or virally infected cells. Interleukin 15 (IL-15) is a critical cytokine for promoting proliferation and the effector capacity of CD8(+) T cells, and has been used to support the growth of CD8(+) T cells in cellular therapies of neoplastic diseases. Recent studies have shown that IL-15, in synergy with other cytokines, such as IL-6, enhances the T-cell receptor (TCR)-independent proliferation and function of CD8(+) T cells. The aim of the present study was to investigate the role of BaF3-mb15-RAE cells in stimulating mouse CD8(+) T cells. BaF3 cells were cultured and B16F10 cells were grown in DMEM. MTT assay was used to detect the proliferation of CD8(+) T cells. Cells were analyzed using flow cytometry. The results showed that IL-15 synergistically acts with another T-cell stimulatory molecule, RAE1ɛ, to potently promote the proliferation of CD8(+) T cells, induce CD8(+) T-cell activation and enhance granzyme B and interferon-γ (IFN-γ) production in the absence of signaling via the TCR. Moreover, IL-15 in combination with RAE1ɛ resulted in a cooperative effect on CD8(+) T-cell-mediated cytotoxicity against B16F10 tumor cells. Thus, results of the present study showed that IL-15, in synergy with RAE1ɛ, enhances the TCR-independent effector function of CD8(+) T cells in vitro, which may be useful in the cellular immunotherapy of cancer.
CD8(+) T细胞通过杀伤恶性或病毒感染细胞在免疫监视中发挥关键作用。白细胞介素15（IL-15）是促进CD8(+) T细胞增殖和效应能力的关键细胞因子，已被用于支持肿瘤疾病细胞治疗中CD8(+) T细胞的生长。最近的研究表明，IL-15与其他细胞因子（如IL-6）协同作用，可增强CD8(+) T细胞不依赖T细胞受体（TCR）的增殖和功能。本研究的目的是探讨BaF3-mb15-RAE细胞在刺激小鼠CD8(+) T细胞中的作用。将BaF3细胞进行培养，B16F10细胞在DMEM中生长。采用MTT法检测CD8(+) T细胞的增殖。使用流式细胞术对细胞进行分析。结果表明，IL-15与另一种T细胞刺激分子RAE1ɛ协同作用，在不通过TCR信号传导的情况下，有力地促进CD8(+) T细胞的增殖，诱导CD8(+) T细胞活化，并增强颗粒酶B和干扰素-γ（IFN-γ）的产生。此外，IL-15与RAE1ɛ联合对CD8(+) T细胞介导的针对B16F10肿瘤细胞的细胞毒性产生协同作用。因此，本研究结果表明，IL-15与RAE1ɛ协同作用可增强体外CD8(+) T细胞不依赖TCR的效应功能，这可能在癌症的细胞免疫治疗中有用。
