Zhao Xudong, Wang Nianfei, Zhang Mingjun, Xue Shaoli, Shi Kaihu, Chen Zhendong
Department of Cardiothoracic Surgery, Second Affiliated Hospital of Anhui Medical University, Hefei, Anhui 230601.
Oncol Lett. 2012 Mar;3(3):654-658. doi: 10.3892/ol.2011.527. Epub 2011 Dec 19.
The aim of this study was to detect methylation of the RAR-β gene in tissues from non-small cell lung cancer (NSCLC) patients. The methylation of the RAR-β gene in DNA from 80 cases with NSCLC and corresponding non-malignant tissues was tested using methylation-specific polymerase chain reaction (PCR; MSP). The results showed that the total frequency of RAR-β methylation was significantly higher in lung cancer tissues compared to the corresponding non-malignant tissues (57.5 vs. 17.5%) (P<0.01). However, no significant difference was found in various clinical stages and types of lung cancer (P>0.05). A significant difference was observed in the various pathological types (P<0.05). RAR-β gene methylation is closely correlated with the development process of NSCLC, particularly squamous cell carcinoma.
本研究的目的是检测非小细胞肺癌（NSCLC）患者组织中RAR-β基因的甲基化情况。采用甲基化特异性聚合酶链反应（PCR；MSP）检测80例NSCLC患者及相应非癌组织DNA中RAR-β基因的甲基化情况。结果显示，与相应的非癌组织相比，肺癌组织中RAR-β甲基化的总频率显著更高（57.5%对17.5%）（P<0.01）。然而，在肺癌的不同临床分期和类型中未发现显著差异（P>0.05）。在不同病理类型中观察到显著差异（P<0.05）。RAR-β基因甲基化与NSCLC的发生发展过程密切相关，尤其是鳞状细胞癌。
