Berclaz Corinne, Goulley Joan, Villiger Martin, Pache Christophe, Bouwens Arno, Martin-Williams Erica, Van de Ville Dimitri, Davison Anthony C, Grapin-Botton Anne, Lasser Theo
Biomed Opt Express. 2012 Jun 1;3(6):1365-80. doi: 10.1364/BOE.3.001365. Epub 2012 May 14.
Diabetes is characterized by hyperglycemia that can result from the loss of pancreatic insulin secreting β-cells in the islets of Langerhans. We analyzed ex vivo the entire gastric and duodenal lobes of a murine pancreas using extended-focus Optical Coherence Microscopy (xfOCM). To identify and quantify the islets of Langerhans observed in xfOCM tomograms we implemented an active contour algorithm based on the level set method. We show that xfOCM reveals a three-dimensional islet distribution consistent with Optical Projection Tomography, albeit with a higher resolution that also enables the detection of the smallest islets (≤ 8000 μm(3)). Although this category of the smallest islets represents only a negligible volume compared to the total β-cell volume, a recent study suggests that these islets, located at the periphery, are the first to be destroyed when type I diabetes develops. Our results underline the capability of xfOCM to contribute to the understanding of the development of diabetes, especially when considering islet volume distribution instead of the total β-cell volume only.
糖尿病的特征是高血糖，这可能是由于胰腺胰岛中分泌胰岛素的β细胞丢失所致。我们使用扩展聚焦光学相干显微镜（xfOCM）对小鼠胰腺的整个胃叶和十二指肠叶进行了离体分析。为了识别和量化在xfOCM断层图像中观察到的胰岛，我们基于水平集方法实施了一种活动轮廓算法。我们表明，xfOCM揭示了与光学投影断层扫描一致的三维胰岛分布，尽管分辨率更高，还能够检测到最小的胰岛（≤8000μm³）。尽管与总的β细胞体积相比，这类最小的胰岛仅占可忽略不计的体积，但最近的一项研究表明，这些位于外周的胰岛在I型糖尿病发生时是最先被破坏的。我们的结果强调了xfOCM有助于理解糖尿病发展的能力，特别是在考虑胰岛体积分布而非仅考虑总的β细胞体积时。
