萘并霉素 L-N，来自链霉菌 CS 的 ansamycin 抗生素。

Naphthomycins L-N, ansamycin antibiotics from Streptomyces sp. CS.

机构信息

State Key Laboratory of Phytochemistry and Plant Resources in West China, Kunming Institute of Botany, Chinese Academy of Sciences , Kunming 650201, People's Republic of China.

出版信息

J Nat Prod. 2012 Jul 27;75(7):1409-13. doi: 10.1021/np300109s. Epub 2012 Jun 28.

DOI:10.1021/np300109s

PMID:22742732

Abstract

Previous analyses of the naphthomycin biosynthetic gene cluster and a comparison with known naphthomycin-type products from Streptomyces sp. CS have suggested that new products can be found from this strain. In this study, screening by LC-MS of Streptomyces sp. CS products formed under different culture conditions revealed several unknown peaks in the product spectra of extracts derived from oatmeal medium cultures. Three new naphthomycins, naphthomycins L (1), M (2), and N (3), and the known naphthomycins A (4), E (5), and D (6) were obtained. The structures were elucidated using spectroscopic data from 1D and 2D NMR and HRESIMS experiments.

摘要

先前对萘莫霉素生物合成基因簇的分析以及与来自链霉菌属 CS 的已知萘莫霉素型产物的比较表明，该菌株可以发现新的产物。在这项研究中，通过 LC-MS 对不同培养条件下形成的链霉菌属 CS 产物进行筛选，在燕麦片培养基培养物提取物的产物谱中发现了几个未知峰。从燕麦片培养基中获得了三种新的萘莫霉素，萘莫霉素 L（1）、M（2）和 N（3），以及已知的萘莫霉素 A（4）、E（5）和 D（6）。通过 1D 和 2D NMR 以及 HRESIMS 实验的光谱数据阐明了它们的结构。