NANOG 和 OCT4 在人类着床前胚胎和人类胚胎干细胞中的共定位。
Co-localization of NANOG and OCT4 in human pre-implantation embryos and in human embryonic stem cells.
机构信息
Department of Clinical Science, Intervention and Technology, Karolinska Institutet, 14186 Stockholm, Sweden.
出版信息
J Assist Reprod Genet. 2012 Oct;29(10):1021-8. doi: 10.1007/s10815-012-9824-9. Epub 2012 Jun 29.
PURPOSE
NANOG and OCT4 are required for the maintenance of pluripotency in embryonic stem cells (ESCs). These proteins are also expressed in the inner cell mass (ICM) of the mouse pre-implantation embryo.
METHODS
Immunohistochemistry was used to show the presence of NANOG and OCT4 protein, and in situ hybridization was used to localize NANOG mRNA in human embryos from two-cell to blastocyst stage, and in human ESCs (hESCs).
RESULTS
Nanog and Oct4 were co-localized in human embryos from morula and blastocyst stages. NANOG mRNA was detected in a group of cells in the morula, in cells of the ICM of blastocysts, and evenly in hESCs. All non-differentiated hESCs expressed NANOG and OCT4 protein. Pluripotent cells expressing NANOG and Oct4 were eccentrically localized, probably in polarized cells in a human compacted morula, which appears to be different from expression in murine embryos.
CONCLUSION
In this study, we demonstrate that whole mount in situ hybridization is amenable to localization of mRNAs in human development, as in other species.
目的
NANOG 和 OCT4 对于维持胚胎干细胞（ESCs）的多能性是必需的。这些蛋白质也在小鼠植入前胚胎的内细胞团（ICM）中表达。
方法
免疫组织化学用于显示 NANOG 和 OCT4 蛋白的存在，原位杂交用于定位从两细胞期到囊胚期的人类胚胎以及人胚胎干细胞（hESCs）中的 NANOG mRNA。
结果
在从桑葚胚和囊胚期的人类胚胎中，Nanog 和 Oct4 共定位。在桑葚胚的一组细胞、囊胚的 ICM 细胞以及 hESCs 中均检测到 NANOG mRNA。所有未分化的 hESCs 均表达 NANOG 和 OCT4 蛋白。表达 NANOG 和 Oct4 的多能细胞呈偏心定位，可能位于人类致密桑葚胚的极化细胞中，这与在鼠胚胎中的表达不同。
结论
在这项研究中，我们证明了整体原位杂交适用于人类发育中 mRNA 的定位，与其他物种相同。
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