Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea.
Proteomics. 2012 Jun;12(12):1999-2013. doi: 10.1002/pmic.201100582.
Recent studies have indicated that obesity increases the risk of developing several types of cancers including lung cancer, which is the leading cause of cancer death worldwide. In the present study, we attempted to discover marker proteins associated with lung cancer progression mediated by treatment of a high-fat diet (HFD) using 2DE combined with MALDI-TOF-MS. Image analysis and further statistical analysis allowed for the detection and identification of 14 proteins, which consequently were classified into two groups based on their regulation patterns in response to diet and tumor. Interestingly, the protein abundances of ten proteins exhibited a synergistic effect when treated with HFD in tumor-bearing mice (Group I). Proteins that had a higher abundance in the plasma of tumor-bearing mice included FGB, Tf, Hpx, Cp, and Hp and the proteins that had a lower abundance included A1AT precursor, PON1, TTRt, and α2-M. These proteins can be used as molecular markers that contribute simultaneously to both obesity and cancer. Four other proteins showed an increase (complement C3 and FGA) or decrease (Apo H and AT III precursor) in the only tumor-bearing mice independently of diet (Group II). The marker proteins identified here may lead to the development of new therapeutics for obesity-causative treatment of lung cancer.
最近的研究表明，肥胖会增加多种癌症的风险，包括肺癌，肺癌是全球癌症死亡的主要原因。在本研究中，我们试图使用 2DE 结合 MALDI-TOF-MS 来发现与高脂肪饮食（HFD）治疗介导的肺癌进展相关的标记蛋白。图像分析和进一步的统计分析允许检测和识别 14 种蛋白质，这些蛋白质根据它们对饮食和肿瘤的反应模式分为两组。有趣的是，当在荷瘤小鼠中用 HFD 处理时，十种蛋白质的蛋白丰度表现出协同作用（第 I 组）。在荷瘤小鼠血浆中丰度较高的蛋白质包括 FGB、Tf、Hpx、Cp 和 Hp，丰度较低的蛋白质包括 A1AT 前体、PON1、TTRt 和 α2-M。这些蛋白质可以作为分子标记物，同时对肥胖和癌症都有贡献。另外四种蛋白质仅在荷瘤小鼠中独立于饮食（第 II 组）增加（补体 C3 和 FGA）或减少（Apo H 和 AT III 前体）。这里鉴定的标记蛋白可能会导致新的治疗肥胖症的药物的开发，从而治疗肺癌。
