Department of Biotechnology, Daegu University, Kyungsan, Kyungbuk, Republic of Korea.
Proteomics. 2010 Dec;10(24):4386-400. doi: 10.1002/pmic.201000391. Epub 2010 Nov 17.
One of the major issues in the field of obesity is why some humans become obese and others resist development of obesity when exposed to high-calorie diets. Despite the same genetic background, namely obesity-prone (OP) and -resistant (OR) rats, differing responses have been demonstrated in a high fat diet-induced rodent model. The aim of the present study was to discover novel obesity-related biomarkers for susceptibility and/or resistance to obesity by proteomic analysis of OP and OR rat plasma. After feeding of high fat diet, OP rats gained approximately 25% more body weight than OR rats and were used for proteomic analysis using 2-DE combined with MALDI-TOF-MS. We categorized identified proteins into three groups by analysis of both average spot density in each group and individual spot density of six rats as a function of body weight. Consequently, category (1) included inter-α-inhibitor H4 heavy chain and fetuin B precursor, which can be used as novel plasma biomarkers for risk of obesity. Nine proteins of category (2) and (3) can also be plausible plasma markers in the study of obesity. This proteomic study is an important advancement over the previous steps needed for identification of OP and OR rats.
肥胖领域的一个主要问题是，为什么有些人在摄入高热量饮食时会肥胖，而有些人则不会。尽管遗传背景相同，即肥胖易感（OP）和肥胖抵抗（OR）大鼠，但在高脂肪饮食诱导的啮齿动物模型中已经表现出不同的反应。本研究的目的是通过肥胖易感和肥胖抵抗大鼠的血浆蛋白质组学分析，发现与肥胖易感性和/或肥胖抵抗相关的新的肥胖相关生物标志物。在给予高脂肪饮食后，OP 大鼠的体重增加了约 25%，比 OR 大鼠多，并用 2-DE 结合 MALDI-TOF-MS 进行蛋白质组学分析。我们通过分析每组平均斑点密度和 6 只大鼠的单个斑点密度，将鉴定出的蛋白质分为三组。结果表明，类别（1）包括α-抑制蛋白 H4 重链和胎球蛋白 B 前体，它们可作为肥胖风险的新型血浆生物标志物。类别（2）和（3）中的 9 种蛋白质也可以作为肥胖研究中的合理血浆标记物。与之前鉴定肥胖易感和肥胖抵抗大鼠所需的步骤相比，这项蛋白质组学研究是一个重要的进步。
