Kippen I, Hirayama B, Klinenberg J R, Wright E M
Biochim Biophys Acta. 1979 Nov 16;558(1):126-35. doi: 10.1016/0005-2736(79)90322-5.
The effect of dibutyryl cyclic AMP on the transport of alpha-methyl-D-glucoside and alpha-aminoisobutyric acid in separated tubules and purified brush border membranes from rabbit kidney was investigated using a rapid filtration procedure. Dibutyryl cyclic AMP stimulated the uptake of alpha-methyl-D-glucoside and alpha-aminoisobutyric acid by separated renal tubules in agreement iwth prior studies utilizing renal slices (Rea, C. and Segal, S. (1973) Biochim. Biophys. Acta 311, 615--624; Weiss, I.W., Morgan, K. and Phang, J.M. (1972) J. Biol. Chem. 247, 760--764). However, in contrast to previous reports, no preincubation of the tissue with dibutyryl cyclic AMP was required for stimulation of transport to be manifest. Dibutyryl cyclic AMP stimulated oxygen consumption by separated tubules suggesting that stimulation of transport may occur by a linkage with renal oxidative metabolism. Dibutyryl cyclic AMP increased the uptake of alpha-aminoisobutyric acid into purified renal brush border membranes. However the uptakes of alpha-methyl-D-glucoside, proline, leucine and phosphate into brush border membranes were significantly inhibited.
采用快速过滤法研究了二丁酰环磷腺苷（dibutyryl cyclic AMP）对兔肾分离肾小管及纯化刷状缘膜中α-甲基-D-葡萄糖苷和α-氨基异丁酸转运的影响。二丁酰环磷腺苷刺激分离的肾小管对α-甲基-D-葡萄糖苷和α-氨基异丁酸的摄取，这与先前利用肾切片进行的研究结果一致（雷亚，C.和西格尔，S.（1973年）《生物化学与生物物理学学报》311卷，615 - 624页；魏斯，I.W.、摩根，K.和方，J.M.（1972年）《生物化学杂志》247卷，760 - 764页）。然而，与先前的报道不同，在刺激转运表现出来之前，不需要用二丁酰环磷腺苷对组织进行预孵育。二丁酰环磷腺苷刺激分离的肾小管耗氧，这表明转运刺激可能通过与肾氧化代谢的联系而发生。二丁酰环磷腺苷增加了α-氨基异丁酸进入纯化肾刷状缘膜的摄取。然而，α-甲基-D-葡萄糖苷、脯氨酸、亮氨酸和磷酸盐进入刷状缘膜的摄取则受到显著抑制。
