Viret J, Leterrier F, Bourre J M
Biochim Biophys Acta. 1979 Nov 16;558(1):141-6. doi: 10.1016/0005-2736(79)90324-9.
The spin labels, 5-nitroxide stearic acid and 16-nitroxide stearic acid were incorporated into whole sciatic nerves dissected from normal, quaking, jimpy and trembler mice. With 5-nitroxide stearic acid, we have studied the thermal variation of the maximal apparent coupling constant (T) between 0 degrees C and 50 degrees C. Within this range of temperatures, we obtained identical values of 2 T for nerves from normal and jimpy mice, whereas 2 T was smaller for nerves from quaking and trembler mice. With 16-nitroxide stearic acid, composite spectra were recorded, particularly in the high-field range. A line characteristic of myelin was clearly observed in the spectra of nerves from normal and jimpy mice; its intensity was somewhat less in nerves from quaking mice and much less in spectra from trembler mice. A shoulder in the principal highfield line of the spectrum is modified only with nerves from jimpy mice. The results agree well with those obtained by electron microscopy, which reveal normal myelination in nerves from jimpy mice, a slight modification of the myelin from those of quaking mice and a practically complete demyelination in peripheral nerves from trembler mice. However, the structure of the nerves of jimpy mice also seems to be modified at an, as yet, undetermined level.
将自旋标记物5-硝基硬脂酸和16-硝基硬脂酸掺入从正常、颤抖、jimpy和震颤小鼠中分离出的完整坐骨神经中。对于5-硝基硬脂酸，我们研究了0℃至50℃之间最大表观耦合常数（T）的热变化。在此温度范围内，我们获得了正常小鼠和jimpy小鼠神经的相同的2T值，而颤抖小鼠和震颤小鼠神经的2T值较小。对于16-硝基硬脂酸，记录了复合光谱，特别是在高场范围内。在正常小鼠和jimpy小鼠神经的光谱中清楚地观察到了髓磷脂的特征线；在颤抖小鼠的神经中其强度有所降低，而在震颤小鼠的光谱中则大大降低。光谱主高场线中的一个肩峰仅在jimpy小鼠的神经中发生改变。这些结果与通过电子显微镜获得的结果非常吻合，电子显微镜显示jimpy小鼠神经中的髓鞘形成正常，颤抖小鼠的髓鞘有轻微改变，而震颤小鼠外周神经中几乎完全脱髓鞘。然而，jimpy小鼠神经的结构似乎在一个尚未确定的水平上也发生了改变。
