Schulte Friederike A, Lambers Floor M, Mueller Thomas L, Stauber Martin, Müller Ralph
a Institute for Biomechanics , ETH Zurich, Wolfgang-Pauli-Strasse 10, 8093 Zurich , Switzerland.
Comput Methods Biomech Biomed Engin. 2014 Apr;17(5):539-48. doi: 10.1080/10255842.2012.699526. Epub 2012 Jun 29.
Time-lapsed in vivo micro-computed tomography is a powerful tool to analyse longitudinal changes in the bone micro-architecture. Registration can overcome problems associated with spatial misalignment between scans; however, it requires image interpolation which might affect the outcome of a subsequent bone morphometric analysis. The impact of the interpolation error itself, though, has not been quantified to date. Therefore, the purpose of this ex vivo study was to elaborate the effect of different interpolator schemes [nearest neighbour, tri-linear and B-spline (BSP)] on bone morphometric indices. None of the interpolator schemes led to significant differences between interpolated and non-interpolated images, with the lowest interpolation error found for BSPs (1.4%). Furthermore, depending on the interpolator, the processing order of registration, Gaussian filtration and binarisation played a role. Independent from the interpolator, the present findings suggest that the evaluation of bone morphometry should be done with images registered using greyscale information.
时间分辨体内微型计算机断层扫描是分析骨微结构纵向变化的有力工具。配准可以克服与扫描之间空间错位相关的问题；然而，它需要图像插值，这可能会影响后续骨形态计量分析的结果。不过，插值误差本身的影响迄今为止尚未量化。因此，本体外研究的目的是阐明不同插值方案（最近邻、三线性和B样条（BSP））对骨形态计量指标的影响。没有一种插值方案导致插值图像和未插值图像之间存在显著差异，其中BSP的插值误差最低（1.4%）。此外，根据插值器的不同，配准、高斯滤波和二值化的处理顺序也起到了作用。与插值器无关，目前的研究结果表明，骨形态计量学的评估应该使用基于灰度信息配准的图像来进行。
