Holmquist R, Moise H
Biochimie. 1979;61(5-6):697-700. doi: 10.1016/s0300-9084(79)80168-6.
During the chromatography of a Triton X-100 extracted preparation of the mitochondrial membrane proteins on diethylaminoethyl cellulose, we have observed two chromatographic fractions containing cytochrome c1. One elutes from diethylaminoethyl cellulose with aqueous buffers alone, and the other elutes with those buffers after the addition of the nonionic detergent Triton X-100. The two forms occur in equimolar ratios and each retains its original chromatographic character on rechromatography, with no conversion of one form into the other.
在用二乙氨基乙基纤维素对经Triton X - 100提取的线粒体膜蛋白制剂进行色谱分析时，我们观察到有两个含有细胞色素c1的色谱级分。一个仅用水性缓冲液就能从二乙氨基乙基纤维素上洗脱下来，另一个则在添加非离子去污剂Triton X - 100后，用这些缓冲液洗脱。这两种形式以等摩尔比存在，并且在重新色谱分析时各自保留其原来的色谱特征，没有一种形式转化为另一种形式。
