Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, NC 27599, USA.
Biomaterials. 2012 Sep;33(27):6305-12. doi: 10.1016/j.biomaterials.2012.05.053. Epub 2012 Jun 27.
Although the release of nitric oxide (NO) from biomaterials has been shown to reduce the foreign body response (FBR), the optimal NO release kinetics and doses remain unknown. Herein, polyurethane-coated wire substrates with varying NO release properties were implanted into porcine subcutaneous tissue for 3, 7, 21 and 42 d. Histological analysis revealed that materials with short NO release durations (i.e., 24 h) were insufficient to reduce the collagen capsule thickness at 3 and 6 weeks, whereas implants with longer release durations (i.e., 3 and 14 d) and greater NO payloads significantly reduced the collagen encapsulation at both 3 and 6 weeks. The acute inflammatory response was mitigated most notably by systems with the longest duration and greatest dose of NO release, supporting the notion that these properties are most critical in circumventing the FBR for subcutaneous biomedical applications (e.g., glucose sensors).
虽然已经证明生物材料中一氧化氮（NO）的释放可以减少异物反应（FBR），但最佳的 NO 释放动力学和剂量仍不清楚。在此，具有不同 NO 释放特性的涂覆有聚氨酯的金属丝植入物被植入猪的皮下组织中 3、7、21 和 42 天。组织学分析表明，NO 释放持续时间较短（即 24 小时）的材料不足以在 3 周和 6 周时降低胶原囊厚度，而释放持续时间较长（即 3 天和 14 天）和 NO 载量较大的植入物则显著减少了 3 周和 6 周时的胶原包裹。具有最长释放时间和最大 NO 剂量的系统显著减轻了急性炎症反应，这支持了这样一种观点，即这些特性对于避免皮下生物医学应用（例如葡萄糖传感器）中的 FBR 最为关键。
