Institute of Hematology and Blood Transfusion, Prague, Czech Republic.
Leuk Res. 2012 Sep;36(9):1128-33. doi: 10.1016/j.leukres.2012.05.012. Epub 2012 Jun 28.
We examined 79 acute myeloid leukemia (AML) patients for DNA methylation of 12 tumor suppressor genes (TSG) and 24 homeobox domain (Hox) genes, and additionally for mutations in DNMT3A gene. We observed lower levels of DNA methylation (P<0.0001) as well as smaller numbers of concurrently hypermethylated genes (P<0.0001) in patients with DNMT3A mutations. Our study of the impact of DNA methylation on prognosis in intermediate and high risk AML patients revealed a relation between higher DNA methylation and better patients' outcome. Lower DNA methylation was linked with higher relapse rates and an inferior overall survival.
我们研究了 79 例急性髓细胞白血病 (AML) 患者的 12 个肿瘤抑制基因 (TSG) 和 24 个同源盒基因 (Hox) 的 DNA 甲基化情况，另外还研究了 DNMT3A 基因突变情况。我们发现，DNMT3A 基因突变患者的 DNA 甲基化水平较低 (P<0.0001)，同时发生超甲基化的基因数量也较少 (P<0.0001)。我们研究了 DNA 甲基化对中高危 AML 患者预后的影响，结果表明 DNA 甲基化水平与患者的预后有关。较高的 DNA 甲基化与较高的复发率和较差的总生存率相关。
