Hesse C J, Baan C C, Daane R, Stolk B J, Jutte N H, Hendriks G F, Weimar W
Department of Internal Medicine I, Erasmus University, Rotterdam, The Netherlands.
Ther Drug Monit. 1990 Nov;12(6):547-53. doi: 10.1097/00007691-199011000-00006.
Four different immunoassays were used to measure cyclosporine A (CsA) plasma (20 degrees C) levels in heart and kidney transplant recipients. Two radioimmunoassays (RIAs) (Sandimmune and Cyclotrac) and the fluorescence polarization immunoassay (FPIA) were based on polyclonal antibodies, whereas the fourth (Cyclotrac-SP) used a CsA-specific mouse monoclonal antibody. We found considerable differences in measured CsA concentrations, which were dependent on the method used and the clinical situation of the patient. Correlation coefficients between the nonspecific assays ranged from 0.899 to 0.901 with plasma values increasing in the order Sandimmune less than Cyclotrac less than FPIA. In the CsA-specific RIA, values were lower, and the correlation with the nonspecific assays ranged from 0.761 to 0.795. In the first 21 days posttransplantation, the heart transplant group showed a higher ratio of nonspecific/specific CsA (mean 4.0) compared with the subsequent period (mean 2.3) or with renal transplant recipients (mean 2.4). The TDX method showed the best assay characteristics. In heart transplant patients with specific and nonspecific 125I-RIA methods, mean CsA levels were 25% lower during rejection periods compared with periods without signs of rejection.
采用四种不同的免疫测定法来检测心脏和肾脏移植受者血浆（20摄氏度）中环孢素A（CsA）的水平。两种放射免疫测定法（RIA）（山地明和环孢素监测仪）以及荧光偏振免疫测定法（FPIA）基于多克隆抗体，而第四种方法（环孢素监测仪 - SP）使用了CsA特异性小鼠单克隆抗体。我们发现所测CsA浓度存在显著差异，这取决于所使用的方法以及患者的临床状况。非特异性测定法之间的相关系数在0.899至0.901之间，血浆值按山地明＜环孢素监测仪＜FPIA的顺序升高。在CsA特异性RIA中，数值较低，与非特异性测定法的相关性在0.761至0.795之间。在移植后的前21天，心脏移植组非特异性/特异性CsA的比例（均值4.0）高于随后时期（均值2.3）或肾移植受者（均值2.4）。TDX方法显示出最佳的测定特性。在采用特异性和非特异性125I - RIA方法的心脏移植患者中，与无排斥迹象的时期相比，排斥期的平均CsA水平低25%。
