Department of Chemical Engineering, National Chung Cheng University, Chia-Yi, Taiwan, ROC.
Colloids Surf B Biointerfaces. 2012 Dec 1;100:9-15. doi: 10.1016/j.colsurfb.2012.05.014. Epub 2012 May 24.
This study presents the differentiation capability of induced pluripotent stem (iPS) cells in heparinized binary scaffolds composed of poly(ε-caprolactone) (PCL) and poly(β-hydroxybutyrate) (PHB). The neuronal differentiation of iPS cells in scaffolds with surface heparin was characterized with immunochemical staining. The results revealed that an increase in the weight percentage of PCL and in the gel concentration enhanced the porosity of scaffolds. Based on the carbon quantity, an increase in the grafting concentration of heparin raised the content of sulfur, nitrogen, and oxygen on the scaffolding surface. In addition, a higher grafting concentration of heparin yielded a weaker expression of stage-specific embryonic antigen 1 in constructs, indicating that the grafted heparin accelerated differentiation and reduced the number of phenotypic iPS cells after cultivation. When the grafting concentration of heparin increased, the staining against βIII tubulin exhibited a stronger fluorescent intensity, demonstrating that iPS cells in constructs differentiated toward neurons. The heparinized PCL-PHB scaffolds can be efficient biomaterials in guiding the differentiation of iPS cells for nerve tissue engineering.
本研究展示了肝素化双组分支架（由聚己内酯（PCL）和聚羟基丁酸酯（PHB）组成）中诱导多能干细胞（iPS）的分化能力。通过免疫化学染色对支架中 iPS 细胞的神经分化进行了特征描述。结果表明，PCL 重量百分比和凝胶浓度的增加均增强了支架的孔隙率。基于碳含量，肝素接枝浓度的增加提高了支架表面的硫、氮和氧含量。此外，较高的肝素接枝浓度导致构建体中胚胎抗原 1 表达较弱，表明接枝的肝素加速了分化，并减少了培养后表型 iPS 细胞的数量。当肝素接枝浓度增加时，βIII 微管蛋白的染色显示出更强的荧光强度，表明构建体中的 iPS 细胞向神经元分化。肝素化 PCL-PHB 支架可作为神经组织工程中指导 iPS 细胞分化的有效生物材料。
