Jiangsu Key Laboratory of Biofunctional Materials, College of Chemistry and Materials Science, Nanjing Normal University, Nanjing 210046, PR China.
Bioelectrochemistry. 2012 Dec;88:1-7. doi: 10.1016/j.bioelechem.2012.05.006. Epub 2012 Jun 7.
The intrinsic properties and application potential of nanocolloids are mainly determined by size, shape, composition, and structure. In this case, a novel glucose biosensor was developed by using the chitosan-polypyrrole (CS-PPy) nanocomposites as special modified materials that coating onto the surface of glassy carbon electrode (GCE). The CS-PPy nanocomposites were characterized by transmission electron microscopy (TEM) and scanning electron microscopy (SEM), respectively. Moreover, the interaction of CS-PPy nanocomposites with glucose oxidase (GOD) was also investigated by the combined studies with Fourier transform infrared spectroscopy (FTIR) and circular dichroism spectroscopy (CD). Due to the conductivity of polypyrrole (PPy), good biocompatibility of CS, and advantages of nanoparticles, CS-PPy nanocomposites were chosen and designed to modify the GCE for the retention of GOD's biological activity and the vantage of electron transfer between GOD and electrodes. The GOD biosensor exhibited a fast amperometric response (5s) to glucose, a good linear current-time relation over a wide range of glucose concentrations from 5.00×10(-4) to 1.47×10(-1)M, and a low detection limit of 1.55×10(-5)M. The GOD biosensor modified with CS-PPy nanocomposites will have essential meaning and practical application in future that attributed to the simple method of fabrication and good performance.
纳米胶体的固有特性和应用潜力主要由其尺寸、形状、组成和结构决定。在这种情况下，通过使用壳聚糖-聚吡咯（CS-PPy）纳米复合材料作为特殊的改性材料，开发了一种新型葡萄糖生物传感器，该材料涂覆在玻碳电极（GCE）的表面。CS-PPy 纳米复合材料分别通过透射电子显微镜（TEM）和扫描电子显微镜（SEM）进行了表征。此外，还通过傅里叶变换红外光谱（FTIR）和圆二色光谱（CD）的联合研究，研究了 CS-PPy 纳米复合材料与葡萄糖氧化酶（GOD）的相互作用。由于聚吡咯（PPy）的导电性、CS 的良好生物相容性和纳米粒子的优势，选择和设计了 CS-PPy 纳米复合材料来修饰 GCE，以保持 GOD 的生物活性和 GOD 与电极之间电子转移的优势。GOD 生物传感器对葡萄糖表现出快速的电流响应（5s），在从 5.00×10(-4)到 1.47×10(-1)M 的宽浓度范围内具有良好的线性电流-时间关系，检测限低至 1.55×10(-5)M。CS-PPy 纳米复合材料修饰的 GOD 生物传感器由于其简单的制备方法和良好的性能，在未来将具有重要的意义和实际应用价值。
