Ebrahimiasl Saeideh, Zakaria Azmi, Kassim Anuar, Basri Sri Norleha
Department of Nanotechnology, Institute of Advanced Technology, Universiti Putra Malaysia, Serdang, Malaysia ; Department of Chemistry, Ahar Branch, Islamic Azad University, Ahar, Iran.
Department of Physics, Universiti Putra Malaysia, Serdang, Malaysia.
Int J Nanomedicine. 2014 Dec 30;10:217-27. doi: 10.2147/IJN.S69740. eCollection 2015.
An antibacterial and conductive bionanocomposite (BNC) film consisting of polypyrrole (Ppy), zinc oxide (ZnO) nanoparticles (NPs), and chitosan (CS) was electrochemically synthesized on indium tin oxide (ITO) glass substrate by electrooxidation of 0.1 M pyrrole in aqueous solution containing appropriate amounts of ZnO NPs uniformly dispersed in CS. This method enables the room temperature electrosynthesis of BNC film consisting of ZnO NPs incorporated within the growing Ppy/CS composite. The morphology of Ppy/ZnO/CS BNC was characterized by scanning electron microscopy. ITO-Ppy/CS and ITO-Ppy/ZnO/CS bioelectrodes were characterized using the Fourier transform infrared technique, X-ray diffraction, and thermogravimetric analysis. The electrical conductivity of nanocomposites was investigated by a four-probe method. The prepared nanocomposites were analyzed for antioxidant activity using the 2,2-diphenyl-1-picrylhydrazyl assay. The results demonstrated that the antioxidant activity of nanocomposites increased remarkably by addition of ZnO NPs. The electrical conductivity of films showed a sudden decrease for lower weight ratios of ZnO NPs (5 wt%), while it was increased gradually for higher ratios (10, 15, and 20 wt%). The nanocomposites were analyzed for antibacterial activity against Gram-positive and Gram-negative bacteria. The results indicated that the synthesized BNC is effective against all of the studied bacteria, and its effectiveness is higher for Pseudomonas aeruginosa. The thermal stability and physical properties of BNC films were increased by an increase in the weight ratio of ZnO NPs, promising novel applications for the electrically conductive polysaccharide-based nanocomposites, particularly those that may exploit the antimicrobial nature of Ppy/ZnO/CS BNCs.
一种由聚吡咯（Ppy）、氧化锌（ZnO）纳米颗粒（NPs）和壳聚糖（CS）组成的抗菌导电生物纳米复合材料（BNC）薄膜，通过在含有均匀分散于CS中的适量ZnO NPs的水溶液中对0.1 M吡咯进行电氧化，在氧化铟锡（ITO）玻璃基板上电化学合成。该方法能够在室温下电合成由生长中的Ppy/CS复合材料中掺入ZnO NPs组成的BNC薄膜。通过扫描电子显微镜对Ppy/ZnO/CS BNC的形态进行了表征。使用傅里叶变换红外技术、X射线衍射和热重分析对ITO-Ppy/CS和ITO-Ppy/ZnO/CS生物电极进行了表征。通过四探针法研究了纳米复合材料的电导率。使用2,2-二苯基-1-苦基肼基测定法分析了制备的纳米复合材料的抗氧化活性。结果表明，添加ZnO NPs后纳米复合材料的抗氧化活性显著提高。对于较低重量比的ZnO NPs（5 wt%），薄膜的电导率突然下降，而对于较高比例（10、15和20 wt%），电导率逐渐增加。分析了纳米复合材料对革兰氏阳性和革兰氏阴性细菌的抗菌活性。结果表明，合成的BNC对所有研究的细菌均有效，对铜绿假单胞菌的有效性更高。随着ZnO NPs重量比的增加，BNC薄膜的热稳定性和物理性能提高，这为基于导电多糖的纳米复合材料带来了新的应用前景，特别是那些可能利用Ppy/ZnO/CS BNCs抗菌性质的应用。
