Himmel Sebastian, Grosse Christian, Wolff Sebastian, Schwiegk Claudia, Becker Stefan
Department of NMR-based Structural Biology, Max Planck Institute for Biophysical Chemistry, Göttingen, Germany.
Acta Crystallogr Sect F Struct Biol Cryst Commun. 2012 Jul 1;68(Pt 7):751-6. doi: 10.1107/S1744309112020635. Epub 2012 Jun 22.
GlcT is a transcriptional antiterminator protein that is involved in regulation of glucose metabolism in Bacillus subtilis. Antiterminator proteins bind specific RNA sequences, thus preventing the formation of overlapping terminator stem-loops. The structure of a fragment (residues 3-170) comprising the RNA-binding domain (RBD) and the first regulatory domain (PRDI) of GlcT was solved at 2.0 Å resolution with one molecule in the asymmetric unit. The two domains are connected by a helical linker. Their interface is mostly constituted by hydrophobic interactions.
葡萄糖转运蛋白（GlcT）是一种转录抗终止蛋白，参与枯草芽孢杆菌葡萄糖代谢的调控。抗终止蛋白结合特定的RNA序列，从而阻止重叠终止子茎环结构的形成。以2.0 Å的分辨率解析了包含GlcT的RNA结合结构域（RBD）和第一个调节结构域（PRDI）的片段（残基3 - 170）的结构，不对称单元中有一个分子。这两个结构域由一个螺旋连接子相连。它们的界面主要由疏水相互作用构成。
